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Preparation of soybean isoflavone aglycones by enzymatic hydrolysis
  
DOI：10.12166/j.zgyz.1003-7969/2020.12.019
KeyWord:soybean isoflavone  glycoside  enzymatic hydrolysis  aglycone
FundProject:中央级公益性科研院所基本科研业务费专项基金（ZX1905）
Author NameAffiliation
ZHOU Wenhong .Institute of Cereal & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 102209, China 
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Abstract:
      Using soybean isoflavone glycosides as raw material, the isoflavone aglycones were prepared by enzymatic hydrolysis. With the hydrolysis rate and aglycones yield as the evaluation indexes, the β-glucosidase, β-galactosidase and cellulase from different sources were screened to determine the optimal hydrolysis enzyme. The enzyme dosage, substrate mass concentration, enzymolysis temperature, pH and enzymolysis time were optimized by single factor experiment. The results showed that the optimal enzymolysis conditions were obtained as follows: β-glucosidase(300 U/g), enzyme dosage 7%, substrate mass concentration 1.6 mg/mL, enzymolysis temperature 56 ℃, pH 4.8, and enzymolysis time 6 h. Under these conditions, the hydrolysis rate of soybean isoflavone glycosides and aglycones yield reached 96.84% and 99.74%, respectively.
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