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| 碳氮比（C/N）对高山被孢霉中花生四烯酸积累的影响 |
| Effects of carbon/nitrogen ratio (C/N) on the arachidonic acid accumulation in Mortierella alpina |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.020 |
| 中文关键词: 高山被孢霉 碳氮比 花生四烯酸 生物量 气相色谱质谱联用技术 |
| 英文关键词:Mortierella alpina C/N arachidonic acid dry cell weight GC-MS |
| 基金项目:云南省农业联合重点项目（2017FG001-015）；云南省自然科学基金面上项目（2016FB030） |
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| 中文摘要: |
| 为探究氮源及碳氮比（C/N）对高山被孢霉（Mortierella alpina）中花生四烯酸（ARA）积累的影响，选取无机氮硝酸钠和有机氮尿素为氮源，分别于高、中、低C/N条件下（C/N为40∶ 1、20∶ 1和10∶ 1）进行M.alpina发酵实验。分析发酵过程中菌体生物量（DCW）、发酵液残C量、发酵液残N量的变化，并通过气相色谱质谱联用技术（GC-MS）检测菌体中脂肪酸组分及其含量的动态变化，同时对各分析指标间进行了Pearson相关性分析。结果表明，以硝酸钠为氮源，高C/N条件下所获DCW更高（6.42 g/L），为低C/N条件下的2.3倍，且高C/N条件下ARA产量也更高（0.21 g/L），高C/N条件下ARA产量与DCW和N消耗量呈显著正相关。以尿素为氮源，低C/N条件下获得较高的DCW（18.2 g/L），但因低C/N条件下菌体中ARA含量较低，故ARA产量不高；且相同C/N条件下，DCW和ARA产量远高于以硝酸钠为氮源的；不同C/N条件下，ARA产量与DCW、C消耗量、N消耗量和总脂肪酸含量均呈显著正相关。综上所述，有机氮比无机氮更有利于M.alpina菌体的生长和ARA的积累，且尿素的中C/N条件更有利于提高ARA的产量（0.99 g/L）。 |
| 英文摘要: |
| In order to investigate the effect of carbon/nitrogen ratio (C/N) on the accumulation of arachidonic acid (ARA) in Mortierella alpina, sodium nitrate (inorganic nitrogen) and urea (organic nitrogen) were selected as the representative nitrogen sources, and M.alpina was fermented at the C/N ratio of 40∶ 1, 20∶ 1 and 10∶ 1, respectively.Changes of dry cell weight (DCW), residual C and N contents in broth during fermentation were measured, the composition and contents of fatty acids in mycelia were also examined by GC-MS, and the correlation analysis of Pearson was carried out between each index.The results showed that with sodium nitrate as the nitrogen source, the DCW （6.42 g/L）obtained under the high C/N condition was significantly higher, which was 2.3 times of that obtained under low C/N, ARA production （0.21 g/L）was also higher, and ARA production was significantly positively correlated with DCW and N consumption under the high C/N condition.With urea as the nitrogen source, the higher DCW（18.2 g/L） was obtained under the low C/N condition, but ARA production was not higher due to the low ARA content in mycelia.Moreover, under the same C/N condition, the DCW and ARA production of mycelia were much higher than those with sodium nitrate as the nitrogen source.With urea as the nitrogen source, ARA production was significantly positively correlated with DCW, C consumption, N consumption and total fatty acid content under different C/N conditions. In summary, organic nitrogen was more beneficial to the growth of M.alpina and ARA accumulation than inorganic nitrogen, and the medium C/N of urea was more conducive to increase the production of ARA（0.99 g/L）.c |
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