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| 氨基化二氧化硅颗粒应用于大豆油DNA提取研究 |
| Application of amino-functionalized silica particles in DNA extraction of soybean oil |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.023 |
| 中文关键词: 氨基化二氧化硅 大豆毛油 DNA提取 PCR扩增 |
| 英文关键词:amino-functionalized silica crude soybean oil DNA extraction PCR amplification |
| 基金项目:苏州市农业农村局为农服务项目；江苏省高等学校大学生创新创业训练项目（201912808001Y）；苏州农业职业技术学院青年教师科研能力提升计划资助项目(19QN1002) |
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| 中文摘要: |
| 建立了以氨基化二氧化硅为载体提取大豆油DNA并进行转基因成分检测的方法。结果表明：利用氨基化二氧化硅法富集提取大豆毛油中的DNA质量浓度高于国标法；以氨基化二氧化硅法提取的大豆毛油DNA为模板成功扩增出了大豆内源基因、外源基因片段和转基因调控元件，而精炼油DNA未扩增出。氨基化二氧化硅法只适用于大豆毛油DNA的提取，而不适用于精炼油。 |
| 英文摘要: |
| A method for extracting DNA from soybean oil using amino-functionalized silica as carrier and detecting transgenic components was established. The results showed that the mass concentration of crude soybean oil DNA extracted by amino-functionalized silica method was higher than that of national standard method, and the crude soybean oil DNA was used as template to successfully amplify the endogenous gene fragment, exogenous gene fragments and transgenic regulatory elements of soybean,while the DNA extracted from refined soybean oil did not amplify the endogenous and exogenous gene fragments. So this method was only applicable to DNA extraction of crude soybean oil, not suitable for DNA extraction of refined soybean oil. |
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