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| Application of amino-functionalized silica particles in DNA extraction of soybean oil |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.023 |
| KeyWord:amino-functionalized silica crude soybean oil DNA extraction PCR amplification |
| FundProject:苏州市农业农村局为农服务项目；江苏省高等学校大学生创新创业训练项目（201912808001Y）；苏州农业职业技术学院青年教师科研能力提升计划资助项目(19QN1002) |
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| Abstract: |
| A method for extracting DNA from soybean oil using amino-functionalized silica as carrier and detecting transgenic components was established. The results showed that the mass concentration of crude soybean oil DNA extracted by amino-functionalized silica method was higher than that of national standard method, and the crude soybean oil DNA was used as template to successfully amplify the endogenous gene fragment, exogenous gene fragments and transgenic regulatory elements of soybean,while the DNA extracted from refined soybean oil did not amplify the endogenous and exogenous gene fragments. So this method was only applicable to DNA extraction of crude soybean oil, not suitable for DNA extraction of refined soybean oil. |
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