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| Determination of glycidyl esters in edible vegetable oil by isotopic internal standard-gas chromatography-mass spectrometry |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.025 |
| KeyWord:glycidyl esters isotopic internal hydrolysis derivatization edible vegetable oil GC-MS |
| FundProject:广东省科学院青年科技工作者引导专项（2019 GDASYL-0105013）；广东省测试分析研究所（中国广州分析测试中心）自筹科研资金项目（201902） |
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| Abstract: |
| An isotopic internal standard-gas chromatography-mass spectrometry method was established for the determination of glycidyl esters (GEs) in edible vegetable oil. GEs were converted to 3-monobromopropanediol (3-MBPD) monoesters, and then the 3-MBPD content was determined by gas chromatography-mass spectrometry (GC-MS) after hydrolysis and derivatization. The conditions of derivatization were optimized. The results showed that the optimal derivatization conditions were obtained as follows: mass concentration of GEs 21.78 μg/mL， phenylboronic acid(PBA) dosage 100 μL, derivatization temperature 50 ℃, derivatization time 10 min. Good linearity was obtained (R=0.999 9) with the mass concentration of GEs in the range of 0.021 8-2.178 μg/mL. The limit of detection of GEs was 25 μg/kg，and the mean recovery rate of standard addition was 91.4%-97.0% with a relative standard deviation of 4.4%-8.6%. The method was applicable and reproducible with good sensitivity, accuracy and precision, which could be used for qualitative and quantitative determination of GEs in edible vegetable oil in actual work. |
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