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| Rapid analysis of cyclolinopeptides by HPLC |
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| DOI：10.12166/j.zgyz.1003-7969/2020.01.027 |
| KeyWord:flaxseed cycolinopeptides high performance liquid chromatography average degree of separation analysis time |
| FundProject:“十三五”国家重点研发计划（2018YFD0401103-2）；国家自然科学青年基金（31701525）；广东省食品副产物增值加工产业技术创新联盟（2017B090907018） |
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| Abstract: |
| The composition and relative content of cyclolinopeptides were analyzed by high performance liquid chromatography (HPLC). HPLC parameters(chromatographic column,injection volume, initial elution volume fraction, flow speed and increase rate of acetonitrile volume fraction) were optimized with the average degree of separation and separation efficiency of 14 kinds of cyclolinopeptide peaks as an index. Cyclolinopeptides were qualitatively determined by HPLC-ESI-MS. The results showed that the optimal HPLC parameter were obtained as follows: Kinetexchromatographic column, water and acetonitrile as the mobile phase, flow rate 1 mL/min, injection volume 5 μL, elution programming of acetonitrile volume fraction increasing from 40% to 90% at 5%/min. 14 peaks of cyclolinopeptides had good separation, and the average degree of separation,analysis time and separation efficiency were 1.65, 9.09 min and 10.86, respectively. The optimal HPLC conditions could be applied in the determination of cyclolinopeptides composition of commercial flaxseed, flaxseed oil and flaxseed meal. |
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