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| 家蝇幼虫耐高温抗氧化蛋白的筛选及其功能研究 |
| Screening and function of thermostable antioxidant protein of Musca domestica larvae |
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| DOI：10.12166/j.zgyz.1003-7969/2020.02.014 |
| 中文关键词: 家蝇幼虫 耐高温 抗氧化 蛋白 H9c2细胞 |
| 英文关键词:Musca domestica larvae thermostable antioxidant protein H9c2 cell |
| 基金项目:广东省科技计划项目(2007B030701006) |
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| 中文摘要: |
| 为寻找耐高温抗氧化蛋白并探究其功能特性，采用60、80、100 ℃处理家蝇幼虫水提取液获得蝇蛆蛋白提取液。通过测定蝇蛆蛋白提取液中蛋白质含量、蛋白质组成以及体外抗氧化活性，确定获得蝇蛆耐高温抗氧化蛋白的最佳处理条件，并对所得蝇蛆耐高温抗氧化蛋白的疏水性以及其对H9c2细胞的氧化损伤保护作用及机理进行了分析。结果表明：高温处理家蝇幼虫水提取液可获得耐高温蛋白，并具有较理想的超氧阴离子自由基、羟自由基清除能力及较高的还原力，100 ℃处理20 min可获得耐高温抗氧化蛋白。该耐高温抗氧化蛋白可使H2O2诱导损伤的H9c2细胞存活率提高约39%，LDH渗出率降低约24%，有效增强细胞的SOD活力、CAT活力及GSH含量（P＜0.05）。研究显示，从蝇蛆中获得了具有良好耐高温特性的抗氧化蛋白，该蛋白对H2O2损伤的H9c2细胞有良好保护作用。 |
| 英文摘要: |
| In order to find thermostable antioxidant proteins and explore their functional properties, the protein extracts from fly maggot were obtained by treating the Musca domestica larvae water extract at 60, 80 ℃ and 100 ℃. By determining the protein content, protein composition and antioxidant activity in the fly maggot protein extract, the optimal treatment conditions for obtaining thermostable antioxidant protein of fly maggot were determined. The hydrophobicity of the thermostable antioxidant proteins of fly maggot, as well as its protective effect on oxidative damage of H9c2 cells and its mechanism were analyzed. The results showed that high temperature treatment of the Musca domestica larvae water extract could obtain thermostable protein, and it had ideal superoxide anion radical, hydroxyl radical scavenging abilities and high reducing power. The optimal treatment condition was 100 ℃ for 20 min.Preliminary analysis of the physiological function of the protein showed that it could increase the survival rate of injured H9c2 cells damaged by hydrogen peroxide by about 39%, and the leakage of lactate dehydrogenase(LDH) decreased by about 24%. In addition, the superoxide dismutase (SOD) activity, catalase (CAT) activity and glutathione (GSH) content were effectively enhanced (P＜0.05). It showed that thermostable antioxidant protein was obtained from fly maggot, and the protein had a good protective effect on H9c2 cells damaged by hydrogen peroxide. |
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