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| Screening and function of thermostable antioxidant protein of Musca domestica larvae |
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| DOI：10.12166/j.zgyz.1003-7969/2020.02.014 |
| KeyWord:Musca domestica larvae thermostable antioxidant protein H9c2 cell |
| FundProject:广东省科技计划项目(2007B030701006) |
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| Abstract: |
| In order to find thermostable antioxidant proteins and explore their functional properties, the protein extracts from fly maggot were obtained by treating the Musca domestica larvae water extract at 60, 80 ℃ and 100 ℃. By determining the protein content, protein composition and antioxidant activity in the fly maggot protein extract, the optimal treatment conditions for obtaining thermostable antioxidant protein of fly maggot were determined. The hydrophobicity of the thermostable antioxidant proteins of fly maggot, as well as its protective effect on oxidative damage of H9c2 cells and its mechanism were analyzed. The results showed that high temperature treatment of the Musca domestica larvae water extract could obtain thermostable protein, and it had ideal superoxide anion radical, hydroxyl radical scavenging abilities and high reducing power. The optimal treatment condition was 100 ℃ for 20 min.Preliminary analysis of the physiological function of the protein showed that it could increase the survival rate of injured H9c2 cells damaged by hydrogen peroxide by about 39%, and the leakage of lactate dehydrogenase(LDH) decreased by about 24%. In addition, the superoxide dismutase (SOD) activity, catalase (CAT) activity and glutathione (GSH) content were effectively enhanced (P＜0.05). It showed that thermostable antioxidant protein was obtained from fly maggot, and the protein had a good protective effect on H9c2 cells damaged by hydrogen peroxide. |
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