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| 油橄榄叶提取物的抑菌活性研究 |
| Antibacterial activity of olive leaves extract |
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| DOI：10.12166/j.zgyz.1003-7969/2020.02.019 |
| 中文关键词: 油橄榄叶 提取物 抑菌活性 细菌 |
| 英文关键词:olive leaf extract antibacterial activity bacteria |
| 基金项目:国家自然科学基金项目(31360192)；2018年陇原青年创新创业团队项目；2017年度西北师范大学青年科技创新团队项目 |
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| 中文摘要: |
| 以牛津杯扩散法考察油橄榄叶提取物(OLE)对大肠杆菌、金黄色葡萄球菌等8种致病菌的抑菌活性，用稀释法分析OLE对8种细菌生长的影响，并探究最低抑菌质量浓度(MIC)和最低杀菌质量浓度(MBC)。结果表明：以盐酸小檗碱作为对照，OLE对蜡状芽孢杆菌、金黄色葡萄球菌、抗四环素大肠杆菌的抑菌作用优于盐酸小檗碱；OLE对表皮葡萄球菌、金黄色葡萄球菌、大肠杆菌的抑菌作用较强（MIC 1 mg/mL，MBC 2 mg/mL）。20 mg/mL的OLE对大肠杆菌的抑制作用最强，为极度敏感(大肠杆菌的d=(25.07±3.02)mm，抗四环素大肠杆菌的d=(24.89±1.58)mm)；葡萄球菌次之(表皮葡萄球菌的d=(20.11±1.02)mm，金黄色葡萄球菌的d=(21.35±206)mm，金黄色葡萄球菌(SH7)d=(20.01±1.21)mm)；而芽孢杆菌处于中度敏感状态。OLE除对铜绿假单胞菌的对数生长期无明显影响外，对其余7种细菌的对数生长期均有延缓作用。OLE具有较强的抑菌活性，可作为一种绿色安全的植物源抑菌制剂。 |
| 英文摘要: |
| The antibacterial activities of olive leaves extract (OLE) against eight pathogenic bacteria including Escherichia coli, Staphylococcus aureus, etc were investigated by Oxford cup diffusion method. The effects of OLE on the growth curve of eight bacteria, as well as the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were analyzed by dilution method. The results showed that using berberine hydrochloride as the control, the inhibitory effects of OLE on Bacillus cereus, Staphylococcus aureus and anti-tetracycline Escherichia coli were better than those of berberine hydrochloride. OLE had strong antibacterial effect on Staphylococcus epidermidis, Staphylococcus aureus and Escherichia coli (MIC 1 mg/mL, MBC 2 mg/mL). OLE of 20 mg/mL had the strongest inhibitory effect on Escherichia coli, and was extremely sensitive (d=(25.07±3.02)mm for Escherichia coli, d=(2489±1.58)mm for anti-tetracycline Escherichic coli), followed by Staphylococcus(d=(20.11±102)mm for Staphylococcus epidermidis, d=(21.35+2.06)mm for Staphylococcus aureus, d=(20.01±121)mm for Staphylococcus aureus (SH7)), while Bacillus was in a moderately sensitive state. Except that OLE had no obvious influence on the logarithmic growth period of Pseudomonas aeruginosa, it could delay the logarithmic growth period of the other seven bacteria. OLE had strong antibacterial activity and could be used as a green, safe and plant-derived bacteriostatic agent. |
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