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| 超声波辅助纤维素酶提取核桃壳多糖及其抗氧化活性研究 |
| Ultrasound-assisted cellulase extraction of polysaccharide from walnut shell and its antioxidant activity |
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| DOI：10.12166/j.zgyz.1003-7969/2020.02.020 |
| 中文关键词: 核桃壳 多糖 超声波辅助 纤维素酶 抗氧化活性 |
| 英文关键词:walnut shell polysaccharide ultrasound-assist cellulase antioxidant activity |
| 基金项目:文山学院科学研究基金 |
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| 中文摘要: |
| 采用单因素试验和正交试验对核桃壳多糖的超声波辅助纤维素酶提取工艺条件进行优化，并对核桃壳多糖的抗氧化活性进行研究。结果表明，超声波辅助纤维素酶提取核桃壳多糖的最优工艺条件为：料液比1∶ 20，纤维素酶添加量1.75%，提取温度45 ℃，提取时间90 min，超声波功率750 W。在最优条件下，核桃壳多糖提取率为2.20%。核桃壳多糖对DPPH自由基、羟基自由基、超氧阴离子自由基均表现出较好的清除能力，且在一定范围内对三者的清除作用呈现良好的量效关系。 |
| 英文摘要: |
| The ultrasound-assisted cellulase extraction conditions of polysaccharide from walnut shell were optimized by single factor experiment and orthogonal experiment, and the antioxidant activity of the polysaccharide was investigated. The results showed that the optimal ultrasound-assisted cellulase extraction conditions were obtained as follows: solid-liquid ratio 1∶ 20, cellulase dosage 1.75%, extraction temperature 45 ℃, extraction time 90 min and ultrasonic power 750 W. Under these conditions, the extraction rate of polysaccharide was 2.20%. The polysaccharide had better scavenging activity on DPPH free radical,·OH and O-2·, and the scavenging rates showed dose-effect relationship to a certain extent. |
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