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| Enzymatic synthesis of soybean sterol oleate |
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| DOI：10.12166/j.zgyz.1003-7969/2020.02.023 |
| KeyWord:soybean sterol oleic acid soybean sterol oleate lipase |
| FundProject:国家自然科学基金项目（31801501）；河南省科技攻关项目（182102110024） |
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| Abstract: |
| Soybean sterol oleate was synthesized from soybean sterol and oleic acid catalyzed by enzyme. The product was qualitatively and quantitatively analyzed by HPLC chromatography. The effects of the type and amount of lipase, the molar ratio of soybean sterol to oleic acid, reaction temperature and reaction time on the yield of soybean sterol oleate were investigated by single factor experiment. The optimal synthesis process of soybean sterol oleate was obtained by orthogonal experiment. The product was characterizated by infrare spectrum. The optimal synthesis conditions of soybean sterol oleate were obtained as follows: with N435 lipase catalyst, molar ratio of soybean sterol to oleic acid 1∶ 1, catalyst dosage 6% (based on total mass of soybean sterol and oleic acid), reaction temperature 50 ℃, reaction time 30 h,isooctane dosage 10 mL(1 mmol of soybean sterol). Under these conditions, the yield of soybean sterol oleate was up to 8651%. The infrared characterization results showed that the synthesized product was soybean sterol oleate. |
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