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| Optimization of aqueous enzymatic extraction process of wax gourd seed oil and its antioxidant activity in vitro |
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| DOI：10.12166/j.zgyz.1003-7969/2020.03.005 |
| KeyWord:wax gourd seed oil aqueous enzymatic method extraction antioxidation activity |
| FundProject:四川省教育厅科研项目（17ZB0324）；2018年地方高校国家级大学生创新创业训练计划项目（201811552027）；川菜发展研究中心项目 |
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| Abstract: |
| Wax gourd seed oil was extracted from wax gourd seed by aqueous enzymatic method. Through single factor experiment the kind of enzyme,pH, enzymolysis time, enzymolysis temperature and ratio of material to liquid on the wax gourd seed oil extraction rate were studied, then the Box-Behnken experiment design was used to determine the optimal extraction conditions. The optimal conditions were obtained by response surface analysis as follows:with dextranase as the enzyme, ratio of material to liquid 1∶ 6,enzymolysis time 6.1 h,dextranase dosage 3%, enzymolysis temperature 60 ℃,pH 4.22. Under the optimal conditions,the wax gourd seed oil extraction rate reached 90.67%.The antioxidant activity in vitro results showed that the DPPH free radical scavenging rate,hydroxyl free radical scavenging rate and iron ion reducing power of wax gourd seed oil increased with the increasing of mass concentration, and the IC50 of DPPH free radical and hydroxyl free radical scavenging rates were 10.23 mg/mL and 0.39 mg/mL,respectively.When the mass concentration of wax gourd seed oil reached 1.0 mg/mL, the hydroxyl free radical scavenging rate reached 98.84%, which was equivalent to the same mass concentration of VC. |
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