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| 水酶法提取冬瓜籽油工艺优化及体外抗氧化活性研究 |
| Optimization of aqueous enzymatic extraction process of wax gourd seed oil and its antioxidant activity in vitro |
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| DOI：10.12166/j.zgyz.1003-7969/2020.03.005 |
| 中文关键词: 冬瓜籽油 水酶法 提取 抗氧化活性 |
| 英文关键词:wax gourd seed oil aqueous enzymatic method extraction antioxidation activity |
| 基金项目:四川省教育厅科研项目（17ZB0324）；2018年地方高校国家级大学生创新创业训练计划项目（201811552027）；川菜发展研究中心项目 |
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| 中文摘要: |
| 以冬瓜籽为原料，采用水酶法提取冬瓜籽油。通过单因素试验设计研究酶的种类、pH、酶解时间、酶解温度、料液比对冬瓜籽油提取率的影响，并利用Box-Behnken试验设计确定冬瓜籽油的最佳提取条件。由响应面分析得出冬瓜籽油的最佳提取条件为：以葡聚糖酶为酶解用酶，料液比1∶ 6，酶解时间6.1 h，酶添加量3%，酶解温度60 ℃，pH 4.22。在最佳提取条件下，冬瓜籽油提取率达90.67%。体外抗氧化活性研究表明：冬瓜籽油对DPPH自由基、羟基自由基清除率及铁离子还原能力随其质量浓度增加而增大；对DPPH自由基清除率IC50为10.23 mg/mL，对羟基自由基清除率IC50为0.39 mg/mL，冬瓜籽油质量浓度为1.0 mg/mL时，其对羟基自由基清除率为98.84%，与VC的清除效果相当。 |
| 英文摘要: |
| Wax gourd seed oil was extracted from wax gourd seed by aqueous enzymatic method. Through single factor experiment the kind of enzyme,pH, enzymolysis time, enzymolysis temperature and ratio of material to liquid on the wax gourd seed oil extraction rate were studied, then the Box-Behnken experiment design was used to determine the optimal extraction conditions. The optimal conditions were obtained by response surface analysis as follows:with dextranase as the enzyme, ratio of material to liquid 1∶ 6,enzymolysis time 6.1 h,dextranase dosage 3%, enzymolysis temperature 60 ℃,pH 4.22. Under the optimal conditions,the wax gourd seed oil extraction rate reached 90.67%.The antioxidant activity in vitro results showed that the DPPH free radical scavenging rate,hydroxyl free radical scavenging rate and iron ion reducing power of wax gourd seed oil increased with the increasing of mass concentration, and the IC50 of DPPH free radical and hydroxyl free radical scavenging rates were 10.23 mg/mL and 0.39 mg/mL,respectively.When the mass concentration of wax gourd seed oil reached 1.0 mg/mL, the hydroxyl free radical scavenging rate reached 98.84%, which was equivalent to the same mass concentration of VC. |
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