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| Determination of benzo\[a\] pyrene in vegetable oil by solid phase extraction and high performance liquid chromatography |
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| DOI：10.12166/j.zgyz.1003-7969/2020.03.010 |
| KeyWord:benzo\[a\]pyrene solid phase extraction high performance liquid chromatography vegetable oil |
| FundProject:大学生创新创业项目（201712709007） |
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| Abstract: |
| A method for determination of benzo\[a\]pyrene using high performance liquid chromatography (HPLC) after a solid phase extraction sample pretreatment was established. The activation and reuse of solid phase extraction column were also studied at the same time. The results showed that the standard calibration curve of benzo\[a\]pyrene had a good linear relationship in the range of 0.5-10.0 μg/L with R2=0.999，the linear equation was y=60 267x+9 951. The detection limit of benzo\[a\]pyrene was 003 μg/kg and the quantitative limit was 0.1 μg/kg. The recovery of the spiked samples with dosage of benzo\[a\]pyrene 0.5-10.0 μg/kg was higher than 90% and the coefficient of variation was less than 5%. In the detection of six different edible oil samples, there was no significant difference between this method and the national standard method（P>0.05）. After a simple reactivation process using ethyl acetate, the extraction column could be reused. Compared with the national standard method, the pretreatment process of this method was simpler and easier to operate, the amount of reagents used was less, the pretreatment time was shorter, the detection limit was lower, and the recovery rate of the method was higher, which met the detection requirements of laboratories and testing organizations. Solid phase extraction column could be reused for five times, which could effectively save the testing cost of a laboratory. |
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