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| 改良QuEChERS-高效液相色谱-串联质谱法检测植物油中的10种植物生长调节剂 |
| Determination of ten plant growth regulators in vegetable oils by improved QuEChERS-high performance liquid |
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| DOI：10.12166/j.zgyz.1003-7969/2020.03.011 |
| 中文关键词: QuEChERS EMR-Lipid 高效液相色谱-串联质谱法 植物油 植物生长调节剂 |
| 英文关键词:QuEChERS EMR-Lipid high performance liquid chromatography-tandem mass spectrometry vegetable oil plant growth regulator |
| 基金项目:国家重点研发计划资助（2018YFC1602800） |
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| 中文摘要: |
| 采用改良QuEChERS技术作为前处理方法,建立了植物油中10种植物生长调节剂（PGRs）的高效液相色谱-串联质谱快速检测方法。样品用酸化乙腈提取，经增强型基质去除吸附剂（EMR-Lipid）净化，然后用高效液相色谱-串联质谱仪多反应监测模式检测，外标法定量。结果表明：方法线性范围1~100 μg/L，10种PGRs线性关系良好，相关系数（r）为0.991~0.998；检出限为0.014~0.188 μg/kg，定量限为0.048~0.625 μg/kg；10种PGRs的回收率在702%~94.7%之间，RSD为1.2%~10.2%。该方法准确、快速、重复性好,适用于植物油中植物生长调节剂的测定 |
| 英文摘要: |
| A rapid method for the simultaneous determination of ten plant growth regulators in vegetable oils was developed by using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) based on modified QuEChERS sample pretreatment method.Samples were extracted with acidic acetonitrile，and cleaned up by Enhanced Matrix Removal Lipid (EMR-Lipid).The analytes were detected in the multi-reaction monitoring (MRM) mode and quantified by the external standard method.The calibration curves of ten plant growth regulators were linear well in the corresponding concentration ranges of 1-100 μg/L, with correlation coefficient（r） of 0.991-0.998. The limits of detection and quantification were 0.014-0.188 μg/kg and 0.048-0.625 μg/kg, respectively. The average recoveries of ten plant growth regulators ranged from 70.2% to 94.7% with relative standard deviations (RSDs) of 1.2%-10.2%.The method was quick,accurate,and sensitive, and was suitable for the determination of ten plant growth regulators in vegetable oils. |
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