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| Response surface optimization of preparing palm residues feed by fermention with Saccharomyces cerevisiae and Lactobacillus |
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| DOI：10.12166/j.zgyz.1003-7969/2020.03.026 |
| KeyWord:Saccharomyces cerevisiae Lactobacillus palm residues feed fermentation response surface methodology |
| FundProject:科技部科技伙伴计划资助（KY201501005）；甘肃省国际科技合作计划项目 (1604WKCA001)；2017年陇原青年创新创业人才(团队)项目(甘组通字〔2017〕121号) |
| Author Name | Affiliation | | LI Bing
JIA Hongzhen
PEER Mohamed Abdul YU Changqing
WU Gang
ZHANG Xiaoxue
WEI Wenhui
LIU Xiaobo
MA Zhongren
DING Gongtao | 1.Separation Technology Development Center, Gansu Light Industry Science Research Institute Co., Ltd.,Lanzhou 730000, China
2.Faculty of Engineering and Built Environment, University Kebangsaan Malaysia,Bangi Selangor 43600, Malaysia
3.Zhangye Olymber Biotechnology Co., Ltd., Zhangye 734000,Gansu, China
4.China-Malaysia National Joint Laboratory, Biomedical Research Center,Northwest Minzu University, Lanzhou 730030, China |
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| Abstract: |
| The single-factor experiment and response surface methodology were used to study the fermentation technology of preparing palm residues feed with Saccharomyces cerevisiae and Lactobacillus. The results showed that the optimal fermentation conditions for the preparation of base material were obtained as follows: fermentation time 5 d, palm kernel residues 70 meshes, ratio of bacterial to material 1∶ 103, fermentation temperature 30 ℃ and water content 36%. Under the optimal conditions, the total number of colonies in the base material was 7.20×1010 colonies/g. The optimal fermentation conditions of the fermentation products were obtained as follows: fermentation temperature 30 ℃, ratio of palm fruit residues to empty fruit bunches 1∶ 2, crushing length of palm funit residues and empty fruit bunches 4 cm, ratio of base material mass to the total mass of palm fruit residues and empty fruit bunches 1∶ 10, water content 35% and fermentation time 36 d. Under the optimal conditions, the OD value of the nutrient in the fermentation product was 0966. The crude protein, crude fat, crude fiber, crude ash, calcium, phosphorus and water contents in the fermentation product were 720%, 761%, 26.20%, 5.20%, 0.38%, 0.26% and 16.20% respectively, and the total number of colonies was 9.0×107 colonies/g.The fermentation method was simple and had good prospects for industrial production. |
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