Effect of lipase on degradation and digestibility of oil and fat in vitro
  
DOI:10.12166/j.zgyz.1003-7969/2020.03.028
KeyWord:oil and fat  lipase  fatty acid  in vitro degradation  digestion
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ZHANG Zhenzhen ZHENG Dawen NING Dong LIU Wenxiu HUANG Xiaotao CHEN Bingfei Asiapac Bio-technology Company Limited, Dongguan 523808Guangdong, China 
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Abstract:
      Using emulsified soyabean oil, rapeseed oil, palm oil, duck fat and lard as raw materials, the hydrolysis effect on the oil and fat added with lipase was determined, the in vitro simulated pig gastrointestinal digestion test was conducted to survey changes of digestibility of piglets added with lipase. The results showed that the emulsified oil and fat reached their degradation peak when reacted for 4 h. The fatty acid produced by different oils and fats reached saturated when added with 200 U/mL lipase(on the basis of oil volume), and fatty acid contents produced by palm oil and lard were higher (P<0.05) than those of soybean oil and duck fat, while rapeseed oil was the lowest. The total amount of increased fatty acid in different emulsified oil and fat groups added with lipase were more than 1.50 mmol/L,as well as total growth rate all exceeded 300%. Even rapeseed oil increased by 810.5%,which was the highest. Moreover, the dry matter digestibility and energy digestibility of piglet added with a 125 g/t lipase(enzyme activity 80 000 U/g) were respectively improved by 0.86 percentage points and 0.83 percentage points during the simulated gastrointestinal digestion of pig. But in consideration of lipid contained in other ingredients, the dosage of lipase slightly above 125 g/t or together with other enzyme were prefered.
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