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| Purification, identification and analysis of malanin from Malania oleifera |
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| DOI：10.12166/j.zgyz.1003-7969/2020.04.006 |
| KeyWord:Malania oleifera malanin isolation and purification |
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| Abstract: |
| Malanin was isolated from Malania oleifera seed kernel by homogenization, extraction with phosphate buffered solution, precipitation by ammonium sulfate and hydrophobic chromatography. The relative molecular weight, amino acid composition, isoelectric point, neutral sugar content, free sulfhydryl content and cell activity were analyzed. The results showed that the malanin was a glycoprotein containing 18 kinds of amino acids, with relative molecular weight of 61 875 Da and isoelectric point of 4.6, and it was linked with two bands of A-chain and B-chain by one or more disulfide bonds. The cytotoxicities of malanin to Hela and Vero cells were very strong. The malanin was a novel protein with good medicinal value. |
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