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Preparation of DHA triglyceride by enzymatic glycerolysis of DHA ethyl ester
  
DOI：10.12166/j.zgyz.1003-7969/2020.04.013
KeyWord:DHA triglyceride  DHA ethyl ester  enzymatic reaction  glycerolysis
FundProject:国家自然科学基金重点项目（31671818）
Author NameAffiliation
LI Zimu BI Yanlan CHENG Yapeng College of Food Science and Technology,Henan University of Technology, Zhengzhou 450001, China 
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Abstract:
      With high purity DHA ethyl ester ( DHA content （78.7±0.6）%) as raw material, DHA triglyceride was prepared by Novozym 435 enzymatic glycerolysis. The effects of molar ratio of DHA ethyl ester to glycerol, reaction temperature, reaction time and dosage of enzyme on the contents of monoglycerides, diglycerides, triglycerides and ethyl ester and conversion rate of ethyl ester in the reaction were investigated. The optimal conditions were obtained as follows: reaction temperature 100 ℃, dosage of enzyme 6% of the mass of DHA ethyl ester, molar ratio of DHA ethyl ester to glycerol 3∶ 1, and reaction time 12 h.Under these conditions, the conversion rate of ethyl ester was (97.0±0.2)％, and the contents of triglycerides and DHA in the product were (95.6±0.1)％ and (76.2±0.0)％ respectively.
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