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| 酶促DHA乙酯甘油解制备DHA甘三酯的工艺研究 |
| Preparation of DHA triglyceride by enzymatic glycerolysis of DHA ethyl ester |
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| DOI：10.12166/j.zgyz.1003-7969/2020.04.013 |
| 中文关键词: DHA甘三酯 DHA乙酯 酶促反应 甘油解 |
| 英文关键词:DHA triglyceride DHA ethyl ester enzymatic reaction glycerolysis |
| 基金项目:国家自然科学基金重点项目（31671818） |
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| 中文摘要: |
| 采用高纯度DHA乙酯（DHA含量（78.7±0.6）%）为原料，在Novozym 435酶的催化作用下甘油解制备DHA甘三酯。考察DHA乙酯与甘油摩尔比、反应温度、反应时间以及加酶量对反应过程中甘一酯、甘二酯、甘三酯、乙酯含量及乙酯转化率的影响，得到最佳条件为反应温度100 ℃、加酶量为DHA乙酯质量的6%、DHA乙酯与甘油摩尔比3∶ 1、反应时间12 h，在此条件下乙酯转化率为（97.0±0.2）%，产物中甘三酯含量为（95.6±0.1）%，DHA含量为（76.2±0.0）%。 |
| 英文摘要: |
| With high purity DHA ethyl ester ( DHA content （78.7±0.6）%) as raw material, DHA triglyceride was prepared by Novozym 435 enzymatic glycerolysis. The effects of molar ratio of DHA ethyl ester to glycerol, reaction temperature, reaction time and dosage of enzyme on the contents of monoglycerides, diglycerides, triglycerides and ethyl ester and conversion rate of ethyl ester in the reaction were investigated. The optimal conditions were obtained as follows: reaction temperature 100 ℃, dosage of enzyme 6% of the mass of DHA ethyl ester, molar ratio of DHA ethyl ester to glycerol 3∶ 1, and reaction time 12 h.Under these conditions, the conversion rate of ethyl ester was (97.0±0.2)％, and the contents of triglycerides and DHA in the product were (95.6±0.1)％ and (76.2±0.0)％ respectively. |
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