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| 樟树籽仁油对高脂诱导型肥胖小鼠体脂和血脂水平的影响 |
| Effect of CCSKO on the body fat and serum lipid levels in obese mice induced by high-fat diet |
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| DOI：10.12166/j.zgyz.1003-7969/2020.04.015 |
| 中文关键词: 樟树籽仁油 降体脂 降血脂 量效关系 |
| 英文关键词:CCSKO reducing body fat reducing serum lipid level dose-effect relationship |
| 基金项目:国家国际科技合作专项项目（2011DFA32770）；江西省科技支持计划重大项目（20143ACG70015）； 2015—2016年度南昌大学食品科学与技术国家重点实验室自由探索课题（SKLF-ZZB-201517）；国家自然科学基金青年科学基金项目（31701651） |
| Author Name | Affiliation | | LI Yanchen
LIN Hui
LI Yitong
ZENG Zheling
YU Ping
WEN Xuefang | 1.School of Resource, Environmental and ChemicalEngineering, Nanchang University, Nanchang 330031,China
2. Jiangxi Province Key Laboratory of Edibleand Medicinal Plant Resources, Nanchang University,
Nanchang 330031, China
3. State Key Laboratory ofFood Science and Technology, Nanchang University,Nanchang 330047, China
4. Institute of AppliedChemistry, Jiangxi Academy of Science, Nanchang
330096, China |
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| 中文摘要: |
| 采用不同樟树籽仁油（CCSKO）含量的高脂饲料干预营养型肥胖小鼠，研究高脂饲料中樟树籽仁油的含量对樟树籽仁油降低营养型肥胖小鼠体脂和血脂效果的影响。正常C57BL/6小鼠饲喂基础饲料作为正常对照组（NC组），同时采用高脂饲料诱发C57BL/6小鼠产生营养型肥胖。将肥胖小鼠随机分为基础饲料恢复组（NR组）、高脂猪油组（HF组）、含21.91%CCSKO（CCSKO替换25%猪油）的高脂饲料组（HF-25%CCSKO组）、含43.83%CCSKO（CCSKO替换50%猪油）的高脂饲料组（HF-50%CCSKO组）、含87.66%CCSKO（CCSKO替换100%猪油）的高脂饲料组（HF-100%CCSKO组），连续喂养10周，测定小鼠体重、体长、腹部脂肪质量及血清相关生化指标，分析肝脏组织切片。结果表明：HF-50%CCSKO组、HF-100%CCSKO组的体重、腹部脂肪质量和血清ALT、AST水平比HF组显著降低，HF-100%CCSKO组的TC、LDL-C、LDL-C/HDL-C水平比HF组显著降低。HF-50%CCSKO组的TC、LDL-C、LDL-C/HDL-C水平与HF组相比，呈现下降趋势。肝脏组织切片显示，HF-50%CCSKO组、HF-100%CCSKO组可减少营养型肥胖小鼠肝脏的脂肪积累，减轻肝细胞脂变程度。CCSKO减少营养型肥胖小鼠体内脂肪积蓄、降低血脂水平效应与高脂饲料中CCSKO含量即CCSKO替换猪油的量呈正相关性。只有当高脂饲料中CCSKO 替代猪油量大于等于50%即CCSKO含量大于等于43.83%时，CCSKO才能显著减少营养型肥胖小鼠体内脂肪沉积、减轻体重、降低血脂水平、修复猪油等LCTs造成的肝脏损伤。CCSKO为天然癸酸月桂酸类MCTs，与辛酸癸酸类MCTs相比较，其减少营养型肥胖小鼠体内脂肪积蓄、降低血脂水平的作用机制，可能既有相同之处，也有不同之处。 |
| 英文摘要: |
| The high fat diet induced obese mice model was used to investigate the body fat and lipid levels reducing effect of Cinnamomum camphora seed kernel oil (CCSKO). The C57BL/6 mice were fed with standard feed as normal control group (NC group). Meanwhile, obese C57BL/6 mice were induced with the high fat diet. The high fat diet induced obese C57BL/6 mice were randomly divided into five groups: naturally restoring group (NR group), high fat diet (HF group), a mixture of lard and CCSKO oil (HF-25%CCSKO group(21.94%CCSKO, m(CCSKO)∶ m(lard)=1∶ 3), HF-50%CCSKO group (4383%CCSKO, m（CCSKO）∶ m（lard）=1∶ 1) and HF-100%CCSKO group (87.66%CCSKO, the lard was completely replaced by CCSKO). The animals were then fed with the above mentioned diets for ten weeks. The body weight, body length, abdominal fat, serum biochemistry parameters as well as liver histological examination were determined at the termination of experiment. The results showed that the body weight, abdominal fat weight, serum ALT and AST in HF-50%CCSKO group and HF-100%CCSKO group were significantly lower than the HF group. The TC, LDL-C, LDL-C/HDL-C ratio in HF-100%CCSKO group were significantly lower compared with the HF group. There was no significant difference between the HF-50%CCSKO group and the HF group in the TC, LDL-C, LDL-C/HDL-C ratio. The results of liver histological examination showed that the fat accumulation and steatosis in the liver were reduced in the HF-CCSKO 50% group and HF-100%CCSKO group. All together, these results suggested that CCSKO had the effect on reducing body fat and serum lipid levels with a dose dependent relationship. The benefits of body fat and serum lipid levels reducing effect could be observed when 50% or more lard in high fat diet was replaced with CCSKO. CCSKO was a capric and lauric MCTs, which was different from commercially available MCTs riched in caprylic acid and decanoic acid. The underlying mechanisms of reducing fat accumulation and lowering blood lipid levels in obese mice by these two types of MCTs might have similarities and differences. |
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