| In order to improve the utilization rate of protein and polyphenols in seabuckthorn(Hippophae rhamnoides L.) seed meal, the extraction process of polyphenols from seabuckthorn(Hippophae rhamnoides L.) seed meal was optimized under the conditions of low protein dissolution rate. At the same time, the components of polyphenols extracted from seabuckthorn(Hippophae rhamnoides L.) seed meal were analyzed by HPLC, and the antioxidant activity of polyphenols was studied. The results showed that the optimal extraction conditions were obtained as follows: 80% methanol solution used as the extraction solvent, ultrasonic extraction method, ultrasonic frequency 40 kHz, ultrasonic power 150 W, ratio of material to liquid 1∶ 25, extraction temperature 20 ℃, extraction time 20 min. Under the optimal extraction conditions, the yield of polyphenols was (7.83±0.03)%. Six polyphenols were detected in the polyphenol extract of seabuckthorn(Hippophae rhamnoides L.) seed meal, including gallic acid, catechin, rutin, myricetin, quercetin and kaempferol. The content of rutin was the highest, which was (1623±0.224) mg/g. The polyphenols had significant scavenging ability on DPPH free radical and hydroxyl free radical, and its IC50 were 0.057 mg/mL and 0147 mg/mL, respectively, which showed that the polyphenols had strong antioxidant activity. |
