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| Extraction and quality analysis of octopus viscera fish oil |
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| DOI：10.12166/j.zgyz.1003-7969/2020.05.004 |
| KeyWord:octopus viscera fish oil extraction process fish oil refining quality analysis |
| FundProject:山东省重点研发计划（2016YYSP003）；青岛市海洋生物医药科技创新中心项目（2017-CXZX01-3-13） |
| Author Name | Affiliation | | FU Xueyuan
ZHONG Hong
SONG Wenshan
LI Bafang | 1.Marine Biomedical Research Institute of Qingdao, Qingdao 266071, Shandong, China
2.Shenzhen Bo Guang Tian Xing Food Co., Ltd., Shenzhen 518000, Guangdong, China
3.Dongshan Bo Guang Tian Xing Food Co., Ltd., Dongshan 363400, Fujian, China) |
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| Abstract: |
| The basic nutritional component, lipid composition and fatty acid composition of octopus viscera were analyzed, and the effects of different extraction methods on oil extraction rate were compared. The method of enzymatic hydrolysis combined with solvent extraction was adopted. In addition, the optimal conditions of enzymolysis were optimized by single factor experiment and orthogonal experiment. The optimal conditions of enzymolysis were obtained as follows: using neutral protease, enzymolysis temperature 50 ℃, ratio of solid to liquid 1∶ 0.5, dosage of enzyme 3 500 U/g, enzymolysis time 4 h. Under these conditions, the oil extraction rate was 74.81%. The refined octopus viscera fish oil was obtained by a series of refining process including degumming, deacidification, cholesterol removal, bleaching and deodorization. The physicochemical properties of the refined octopus viscera fish oil basically reached the second grade standard of refined fish oil (SC/T 3502—2016), and no obvious change was observed in fatty acid composition of the fish oil. The polyunsaturated fatty acid content of the refined fish oil was 50.51%, and the total content of EPA and DHA was 37.30%. Octopus viscera fish oil had high application value and development prospect. |
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