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| Optimization of subcritical extraction of Nannochloropsis lipid and its component analysis |
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| DOI：10.12166/j.zgyz.1003-7969/2020.05.005 |
| KeyWord:subcritical butane Nannochloropsis lipid physicochemical index phospholipid fatty acid |
| FundProject:“十三五”国家重点研发计划支持项目（2018YFD 0401105-03） |
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| Abstract: |
| The Nannochloropsis lipid was extracted by subcritical butane. The factors affecting extraction rate of Nannochloropsis lipid were optimized by orthogonal experiment on the basis of single factor experiment.The acid value, peroxide value, iodine value, phospholipid content, fat composition and fatty acid composition of the lipid were determined.The results showed that the optimal conditions were obtained as follows: single extraction time 40 min, extraction times 4, extraction temperature 45 ℃and ratio of solid to solvent 1∶ 8. Under these conditions, the extraction rate of Nannochloropsis lipid was 4155%.The extracted Nannochloropsis lipid was a dark green oily paste, with the acid value 20.4 mgKOH/g, peroxide value 0.001 g/100 g, iodine value 86.0 gI/100 g and phospholipid content 107.28 mg/g. The total fat content in the lipid was 40.22%, and the fatty acids in the lipid of Nannochloropsis were mainly unsaturated fatty acids with the EPA content 7.21%. |
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