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| 体外模拟消化芝麻蛋白产生多肽的抗氧化性分析 |
| Antioxidant analysis of peptides produced by in vitro simulated digestion of sesame protein |
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| DOI：10.12166/j.zgyz.1003-7969/2020.05.013 |
| 中文关键词: 芝麻 芝麻蛋白 抗氧化肽 体外模拟消化 胃蛋白酶 胰蛋白酶 |
| 英文关键词:sesame sesame protein antioxidant peptides in vitro simulated digestion pepsin trypsin |
| 基金项目:河南省农业科学院自主创新专项基金项目(2019ZC54)；国家特色油料产业技术体系（CARS-14） |
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| 中文摘要: |
| 为证实芝麻蛋白在人体内消化可产生抗氧化肽，采用体外模拟消化芝麻蛋白，分析芝麻蛋白水解产生多肽的组成与抗氧化活性。结果显示：在体外模拟消化模型中，芝麻蛋白在1 h内丧失原有亚基结构，最终水解为相对分子质量在15 kDa以下的多肽；水解产生的多肽具有抗氧化性，其中胃蛋白酶水解产生的多肽具有较强的DPPH自由基清除能力，而胰蛋白酶与胰凝乳蛋白酶的水解有助于提升多肽的ABTS自由基清除能力。上述结果表明，芝麻蛋白在人体内消化会产生抗氧化肽，不同消化阶段的芝麻多肽组成有差异，从而造成抗氧化活性的差别。 |
| 英文摘要: |
| In order to confirm sesame protein could generate antioxidant peptides during gastrointestinal digestion, in vitro simulated digestion of sesame protein was used to analyze changes of peptides composition and antioxidant activity. The results showed that sesame protein lost its original subunit structure within 1 h, and was finally hydrolyzed into peptides with the relative molecular weight below 15 kDa. Sesame peptides produced by in vitro simulated digestion displayed antioxidant activity. Peptides hydrolyzed by pepsin possessed higher DPPH free radical scavenging activity, while hydrolysis of trypsin and chymotrypsin could improve the ABTS free radical scavenging activity of peptides. According to above results, sesame protein could generate antioxidant peptides in the gastrointestinal digestion, and compositions of sesame peptides were various during different digestion stages, as a result, there were some differences in their antioxidant activity. |
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