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| Screening of a desert oil-producing microalgae and its growth and oil production response toward pH |
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| DOI：10.12166/j.zgyz.1003-7969/2020.05.017 |
| KeyWord:desert oil-producing microalgae screening pH growth oil production |
| FundProject:安徽工程大学“中青年拔尖人才培养计划”资助项目（S022019007）；安徽工程大学人才启动基金项目（2018YQQ030）；国家自然科学基金项目（21666034） |
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| Abstract: |
| A strain of oil-producing microalgae DT025 was obtained by isolating after being enriched and cultured from water samples collected from salt marsh in Xinjiang desert. The growth characteristics and oil accumulation characteristics of the microalgae DT025 were analyzed, and molecular biology identification was performed. In addition, the effects of different pH on the growth and oil production of the microalgae DT025 were discussed. The results showed that the microalgal strain was identified as Chlorella sp., and exhibited good environmental adaptability and oil accumulation ability, which grew rapidly since culturing for 2 d, and began to accumulate abundant oil since culturing for 4 d, as a result, the maximum oil content of 37.13% was obtained when cultured for 8 d. The obtained microalgae possessed good pH tolerance, which could grow well in the pH rang of 6.0-9.0, and the suitable pH range for oil production was 7.0-8.0. The maximum oil production and yield of the microalgae were obtained at pH 7.5, which were 1.580 g/L and 0.198 g/(L·d) respectively. Besides, the relatively high oil production and yield were also obtained at pH 8.0 and 8.5, which reached 1.460 g/L, 0.183 g/(L·d) and 0.859 g/L,0107 g/(L·d), respectively, indicating the good alkali resistance and oil production capacity of the microalgae. |
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