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| Optimization of enzymolysis production of Millettia dielsiana antioxidant peptide by response surface methodology |
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| DOI：10.12166/j.zgyz.1003-7969/2020.06.007 |
| KeyWord:Millettia dielsiana antioxidant peptide salt soluble protein pepsin enzymolysis DPPH free radical scavenging rate |
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| Abstract: |
| Using Millettia dielsiana as raw material, the protein was extracted by salt extraction method and water extraction method respectively. Comparing the DPPH free radical scavenging ability of salt soluble protein and water soluble protein, it was concluded that the DPPH free radical scavenging ability of salt soluble protein was higher.Then,with Millettia dielsiana salt soluble protein as material, enzymolysis production process of Millettia dielsiana antioxidant peptide was optimized by response surface methodology. The results showed that pepsin was selected as the best enzyme with DPPH free radical scavenging rate as index. The optimal conditions for preparing Millettia dielsiana antioxidant peptide were determined as follows:substrate mass concentration 0.8 mg/mL,enzymolysis temperature 34 ℃, enzyme amount 1 100 U/g, enzymolysis time 36 min, and enzymolysis pH 2.0.Under the optimal conditions, the DPPH free radical scavenging rate of Millettia dielsiana antioxidant peptide was 70.41% and the yield of polypeptide was 53.63%. |
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