| 1,3-dioleoyl-2-palmitoyl glycerol(OPO) is an important component of human milk fat, which can promote the absorption and utilization of fatty acids in infants. With high oleic acid peanut oil fatty acid ethyl ester(ethyl oleate) and palm stearin as raw materials, OPO was synthesized by transesterification under the catalysis of Novozym 40086 lipase.The process conditions for enzymatic synthesis of OPO were optimized by single factor experiment and response surface methodology. The results showed that the optimal reaction conditions were obtained as follows: molar ratio of ethyl oleate to palm stearin 6∶ 1, reaction time 1 h, reaction temperature 60 ℃, and dosage of enzyme 8%(based on total substrate mass). Under these conditions, the OPO content, contents of 2-palmitic acid accounted for all palmitic acid and PPP in triglyceride of the product were(46.30±0.03)%,(60.70±0.23)% and(5.67±030)%, respectively. All the product indexes met the national standard GB 30604—2015. |
