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| 酶促油酸乙酯与棕榈硬脂酯交换合成OPO工艺研究 |
| Synthesis of OPO by enzymatic transesterification of ethyl oleate with palm stearin |
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| DOI：10.12166/j.zgyz.1003-7969/2020.06.017 |
| 中文关键词: 高油酸花生油脂肪酸乙酯 油酸乙酯 棕榈硬脂 酶法酯交换 1,3-二油酸-2-棕榈酸甘油三酯 Novozym 40086脂肪酶 |
| 英文关键词:high oleic acid peanut oil fatty acid ethyl ester ethyl oleate palm stearin enzymatic transesterification 1,3-dioleoyl-2-palmitoyl glycerol Novozym 40086 lipase |
| 基金项目:国家自然科学基金重点项目（31671818） |
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| 中文摘要: |
| 1,3-二油酸-2-棕榈酸甘油三酯（OPO）是人乳脂中重要组成部分，能促进婴儿对脂肪酸的吸收利用。以高油酸花生油脂肪酸乙酯（以下简称油酸乙酯）和棕榈硬脂为原料，在Novozym 40086脂肪酶的催化作用下酯交换合成OPO。在单因素实验的基础上采用响应面法对酶法合成OPO的工艺条件进行优化。结果表明：酶法合成OPO的最佳反应条件为油酸乙酯与棕榈硬脂摩尔比6∶ 1、反应时间1 h、反应温度60 ℃、加酶量8%（以底物总质量计），在该条件下合成的产物甘三酯中OPO含量、2位棕榈酸占所有棕榈酸含量以及PPP含量分别为（46.30±0.03）%、（60.70±023）%、（5.67±0.30）%。产品指标均满足GB 30604—2015的规定。 |
| 英文摘要: |
| 1,3-dioleoyl-2-palmitoyl glycerol(OPO) is an important component of human milk fat, which can promote the absorption and utilization of fatty acids in infants. With high oleic acid peanut oil fatty acid ethyl ester(ethyl oleate) and palm stearin as raw materials, OPO was synthesized by transesterification under the catalysis of Novozym 40086 lipase.The process conditions for enzymatic synthesis of OPO were optimized by single factor experiment and response surface methodology. The results showed that the optimal reaction conditions were obtained as follows: molar ratio of ethyl oleate to palm stearin 6∶ 1, reaction time 1 h, reaction temperature 60 ℃, and dosage of enzyme 8%(based on total substrate mass). Under these conditions, the OPO content, contents of 2-palmitic acid accounted for all palmitic acid and PPP in triglyceride of the product were(46.30±0.03)%,(60.70±0.23)% and(5.67±030)%, respectively. All the product indexes met the national standard GB 30604—2015. |
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