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| Preparation of pumpkin seed polypeptide and its effects on human skin cells in vitro |
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| DOI：10.12166/j.zgyz.1003-7969/2020.06.020 |
| KeyWord:pumpkin seed polypeptides enzymatic hydrolysis cell proliferation reactive oxygen species |
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| Abstract: |
| Using pumpkin seeds as raw materials, pumpkin seed protein was prepared. Five polypeptides were prepared respectively by enzymatic hydrolysis of pumpkin seed protein with alkaline protease, trypsin, flavor protease, alkaline protease-trypsin and alkaline protease-flavor protease. The results showed that among the five hydrolysates, the degree of hydrolysis (DH) and the yield of polypeptide with alkaline protease-trypsin were the highest (the DH value 27.23% and the yield of polypeptide 39.20%). Because the content of less than 1 000 Da polypeptide in the pumpkin seed polypeptide prepared by alkaline protease-trypsin was as high as 92.72%, so it had a good performance in promoting human skin cell proliferation. In addition, it could scavenge free radicals and reduce the content of intracellular reactive oxygen species, so it could repair cells when human skin cells were damaged by oxidation. |
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