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| Simultaneous determination of lignans and tocopherols in sesame products by high performance liquid chromatography |
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| DOI：10.12166/j.zgyz.1003-7969/2020.06.026 |
| KeyWord:sesame oil sesame paste high performance liquid chromatography lignans tocopherol |
| FundProject:山东省农业重大应用技术创新项目（SD2019ZZ024） |
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| Abstract: |
| A method for simultaneous determination of three kinds of lignans and tocopherols in sesame products by high performance liquid chromatography was established. Sesame oil and black-and white-sesame paste were used as raw materials to optimize the ultrasound pretreatment conditions. The samples were extracted by ultrasonic assisted methanol. The gradient elution was carried out using Symmetry C18 column and methanol - water as mobile phase. The results showed that the optimal ultrasound pretreatment conditions were obtained as follows: ultrasonic power 320 W, ratio of material to liquid 1∶ 120(sesame oil), ratio of material to liquid 1∶ 80(sesame paste) and ultrasonic time 20 min. The average recovery, precision, detection limit and quantitation limit of lignans and tocopherols were 88.5%-108.4%, 24%-4.8%,0.43-0.98 mg/kg and 1.33-2.84 mg/kg respectively. The separation effect of lignans and tocopherols was good. |
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