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| 高效液相色谱法同时测定芝麻制品中木脂素及生育酚含量的研究 |
| Simultaneous determination of lignans and tocopherols in sesame products by high performance liquid chromatography |
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| DOI：10.12166/j.zgyz.1003-7969/2020.06.026 |
| 中文关键词: 芝麻油 芝麻酱 高效液相色谱 木脂素 生育酚 |
| 英文关键词:sesame oil sesame paste high performance liquid chromatography lignans tocopherol |
| 基金项目:山东省农业重大应用技术创新项目（SD2019ZZ024） |
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| 中文摘要: |
| 建立了高效液相色谱法同时测定芝麻制品中3种木脂素及生育酚含量的研究方法。以市售芝麻油和黑、白芝麻酱为原料，优化了超声波前处理条件，样品经超声波辅助甲醇萃取后，利用Symmetry C18色谱分离柱，以甲醇-水为流动相进行梯度洗脱。结果表明：超声波前处理最佳条件为超声波功率320 W、料液比1∶ 120(芝麻油)、料液比1∶ 80（芝麻酱）、超声时间20 min；3种木脂素和生育酚平均回收率在88.5%～108.4%之间，精密度在2.4%～4.8%之间，检出限为0.43~0.98 mg/kg，定量限为1.33~2.84 mg/kg，木脂素及生育酚类化合物分离效果好。 |
| 英文摘要: |
| A method for simultaneous determination of three kinds of lignans and tocopherols in sesame products by high performance liquid chromatography was established. Sesame oil and black-and white-sesame paste were used as raw materials to optimize the ultrasound pretreatment conditions. The samples were extracted by ultrasonic assisted methanol. The gradient elution was carried out using Symmetry C18 column and methanol - water as mobile phase. The results showed that the optimal ultrasound pretreatment conditions were obtained as follows: ultrasonic power 320 W, ratio of material to liquid 1∶ 120(sesame oil), ratio of material to liquid 1∶ 80(sesame paste) and ultrasonic time 20 min. The average recovery, precision, detection limit and quantitation limit of lignans and tocopherols were 88.5%-108.4%, 24%-4.8%,0.43-0.98 mg/kg and 1.33-2.84 mg/kg respectively. The separation effect of lignans and tocopherols was good. |
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