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| 黑芝麻总黄酮的体内抗氧化作用研究 |
| Antioxidation in vivo of total flavonoids from black sesame |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.010 |
| 中文关键词: 黑芝麻 总黄酮 抗氧化 衰老模型 |
| 英文关键词:black sesame total flavonoids antioxidation aging model |
| 基金项目:西安医学院药学省级重点学科建设项目（2016YXXK09，2016YXXK16）；西安市未央区科技计划项目（201930）；西安医学院国家自然科学基金培育项目（2017GJFY13）；国家自然科学基金（81903288）;西安市科技计划项目（2020KJRC0135） |
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| 中文摘要: |
| 为了评价黑芝麻总黄酮的体内抗氧化活性，通过颈部皮下注射D-半乳糖建立小鼠衰老模型，灌胃给药，用转棒式疲劳仪和负重游泳实验检测小鼠运动能力，计算胸腺、脾脏及脑指数，分别检测小鼠血清和脑组织匀浆液中丙二醛（MDA）含量、总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活力，HE染色法观察脑组织病理变化。结果表明：与衰老模型小鼠相比，黑芝麻总黄酮高剂量组可显著延长小鼠负重游泳时间，增加脑指数（P<0.05），显著增加血清中T-SOD和GSH-Px活力，降低MDA含量（P<0.05）；黑芝麻总黄酮高、中剂量组能显著升高脑组织中T-SOD的活力，降低MDA含量（P<0.05）；HE染色结果显示黑芝麻总黄酮高剂量组能改善脑组织损伤程度。黑芝麻总黄酮具有抗衰老活性，其可能通过抗氧化活性发挥抗衰老作用。 |
| 英文摘要: |
| To evaluate the antioxidation in vivo of total flavonoids from black sesame, the aging model mice were induced by subcutaneous injection of D-galactose at neck back, and intragastrily administrated with total flavonoids from black sesame. Behavioral changes of mice were evaluated by rotary rod fatigue tester and weight-bearing swimming test. The indexes of thymus, spleen and brain were calculated. The activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) content in serum and brain tissue homogenate were detected. The pathological morphological changes in brain tissue were observed by HE staining. The results showed that compared with the model group the high dose of total flavonoids from black sesame could significantly prolong the weight-bearing swimming time, significantly increase brain index and the activities of T-SOD and GSH-Px (P<0.05), and decrease MDA content in serum (P<0.05). The high and medium dose of total flavonoids from black sesame could significantly increase the activity of T-SOD in brain tissue, and reduce MDA content (P<0.05). HE staining results showed that the high dose of total flavonoids from black sesame could relieve brain tissue damage.The total flavonoids from black sesame had anti-aging activity via antioxidant capacity. |
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