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| Carboxymethylation modification of Camellia oleifera seed polysaccharides and its antioxidant effect on oils and fats |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.011 |
| KeyWord:Camellia oleifera seed polysaccharides carboxymethylation specific scavenging rate oils and fats antioxidation |
| FundProject:江西省教育厅科学技术研究项目（GJJ161055） |
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| Abstract: |
| The conditions of Camellia oleifera seed polysaccharides (COSP) modified by carboxymethylation were optimized in the NaOH and sodium chloroacetate reaction system with the specific scavenging rate of COSP carboxymethylation(COSPC) on O-2· as an index. The antioxidant effect of COSPC on oils and fats was investigated. The effects of ethanol volume fraction, mass ratio of COSP to NaOH and sodium chloroacetate, reaction temperature and reaction time on specific scavenging rate of COSPC on O-2· were analysed by single factor experiment and orthogonal experiment. The antioxidant effects of COSPC on oils and fats were investigated by Schaal oven method. The results showed that the optimal conditions of COSP modified by carboxymethylation were obtained as follows: ethanol volume fraction 80%, mass ratio of COSP to NaOH and sodium chloroacetate 1∶ 3∶ 2, reaction temperature 50 ℃ and reaction time 3 h. Under these conditions, the specific scavenging rate of COSPC on O-2· was 0.600 mL/mg and the substitution degree was 0.624. The antioxidant effect of COSPC on oils and fats was significantly higher than that of COSP. The carboxymethylation modification of COSP could improve its antioxidant effect. |
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