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| Effect of simulate human gastrointestinal biomimetic digestion on antioxidant ability of peanut peptide-ferrous |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.012 |
| KeyWord:peanut peptide-ferrous(PPF) gastrointestinal biomimetic digestion reactive oxygen species (ROS) linoleic acid oxidation liposome peroxidation |
| FundProject:湖南省自然科学基金科教联合基金项目（2018JJ5022）；湖南省教育厅科研项目（18C1356） |
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| Abstract: |
| To investigate the changing rule of antioxidant activity of peanut peptide-ferrous (PPF) during simulated human gastrointestinal digestion (SHGD), the surface hydrophobicity, DPPH· scavenging rate, O-2 ·scavenging rate, ·OH scavenging rate, linoleic acid oxidation inhibitory activity and lipid peroxidation inhibitory activity of PPF during SHGD were studied. The results showed that SHGD enhanced the surface hydrophobicity, and the surface hydrophobicity of (15.84±0.33) μg was the highest at 270 min. SHGD enhanced the DPPH· scavenging capacity, the DPPH· scavenging rate was (88.77±1.00)% at 300 min, which was significantly higher than that of 1.0 mg/mL VC. The scavenging capacities on O-2· and ·OH of PPF gastrointestinal biomimetic digestive products were markedly weaker than that of 1.0 mg/mL VC and 0.6 mg/mL VC, respectively. The gastrointestinal biomimetic digestion product had better linoleic acid oxidation inhibition effect, and the intestinal biomimetic digestion products showed the highest inhibitory acitivity. Biomimetic digestion also enhanced the protection of lipid peroxidation, and the inhibition rate of lipid peroxidation was the highest at 330 min and 20 mg/L, which was significantly lower than that of VC. The results indicated that SHGD enhanced the antioxidant activity of PPF. |
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