| The Eversa Transform 2.0 lipase is cheap and the catalytic activity is good, so it has the advantage of micro-high efficiency. The enzymatic methyl esterification process of soybean oil deodorized distillate was studied with Eversa Transform 2.0 lipase as catalyst.The effects of molar ratio of alcohol to acid, enzyme dosage, reaction temperature and reaction time on esterification rate were studied by single factor experiment. The orthogonal experiment was carried out to optimize the enzymatic methyl esterification process.Under the optimal process conditions, the test results were verified by amplification test, and the conversion of triglyceride was analyzed by thin layer chromatography.The results showed that the optimal conditions for the methyl esterification reaction of soybean oil deodorized distillate catalyzed by Eversa Transform 2.0 lipase were obtained as follows: molar ratio of methanol to free fatty acid 2∶ 1, enzyme dosage 0.3% (relative to the mass of soybean oil deodorized distillate), reaction temperature 35 ℃, reaction time 6 h.Under these conditions, the esterification rate reached above 95%, and the triglyceride was almost completely reacted. |
