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| Influencing factors on growth and lipid production of Mortierella alpina |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.027 |
| KeyWord:Mortierella alpina culture condition growth lipid production |
| FundProject:国家自然科学基金青年基金（31901659） |
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| Abstract: |
| In order to analyze the factors affecting growth and lipid production of Mortierella alpina, the culture conditions including initial glucose mass concentration, activation times, inoculation size, loaded liquid, fermentation time, and breaking time of pellet were studied. The results showed that initial glucose mass concentration, loaded liquid and fermentation time had the most significant effect, and the initial glucose mass concentration had negative correlation with the production of arachidonic acid. Based on the growth and lipid production of this strain under different culture conditions, the optimal culture conditions were obtained as follows: the strain was activated for three times and broken for 20 s at 8 000 r/min to a uniform state, and then added into the broth fermentation medium with 2%-3% (volume fraction) inoculation amount, cultured in fermentation medium with 50 g/L initial glucose mass concentration and 20% (volume fraction) loaded liquid, then cultured at 200 r/min, 28 ℃ for 8.5 d. |
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