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| 高山被孢霉生长和产脂的影响因素分析 |
| Influencing factors on growth and lipid production of Mortierella alpina |
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| DOI：10.12166/j.zgyz.1003-7969/2020.07.027 |
| 中文关键词: 高山被孢霉 培养条件 生长 产脂 |
| 英文关键词:Mortierella alpina culture condition growth lipid production |
| 基金项目:国家自然科学基金青年基金（31901659） |
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| 中文摘要: |
| 为探究影响高山被孢霉生长和产脂的因素，分析不同初始葡萄糖质量浓度、活化次数、接种量、装液量、发酵时间、菌球打碎时间等条件下高山被孢霉生长和产脂特点。结果表明，初始葡萄糖质量浓度、装液量和发酵时间对菌株生长和产脂的影响最为显著，且初始葡萄糖质量浓度与花生四烯酸的产量呈负相关性。根据不同条件下菌株生长和产脂情况，得到高山被孢霉最佳培养条件为：菌株活化3次，用高速分散机8 000 r/min打碎20 s至均匀状态，按2%~3%（体积分数）接种量接入初始葡萄糖质量浓度为50 g/L、装液量为20%（体积分数）的发酵培养基中，200 r/min、28 ℃培养85 d。 |
| 英文摘要: |
| In order to analyze the factors affecting growth and lipid production of Mortierella alpina, the culture conditions including initial glucose mass concentration, activation times, inoculation size, loaded liquid, fermentation time, and breaking time of pellet were studied. The results showed that initial glucose mass concentration, loaded liquid and fermentation time had the most significant effect, and the initial glucose mass concentration had negative correlation with the production of arachidonic acid. Based on the growth and lipid production of this strain under different culture conditions, the optimal culture conditions were obtained as follows: the strain was activated for three times and broken for 20 s at 8 000 r/min to a uniform state, and then added into the broth fermentation medium with 2%-3% (volume fraction) inoculation amount, cultured in fermentation medium with 50 g/L initial glucose mass concentration and 20% (volume fraction) loaded liquid, then cultured at 200 r/min, 28 ℃ for 8.5 d. |
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