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| Anti-Parkinsons activity of peony seed oil microcapsule powder based on zebrafish Parkinsons desease model |
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| DOI：10.12166/j.zgyz.1003-7969/2020.08.010 |
| KeyWord:：peony seed oil peony seed oil microcapsule powder zebrafish Parkinsons desease |
| FundProject:山东省重点研发计划（2017YYSP032）；山西医科大学汾阳学院人才引进启动金基金项目（2016D03） |
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| Abstract: |
| In order to investigate whether peony seed oil microcapsule powder had anti-Parkinsons activity, different experiment groups were set including the control group, 50 μmol/L MPTP treatment group, and co-treatment groups (25, 50,100 μg/mL) of peony seed oil microcapsule powder with 50 μmol/L MPTP. Transgenic zebrafish Vmat:GFP, Fli1:GFP embryos, and wild-type zebrafish AB embryos were treated at 1 day after fertilization(1 dpf). At 3 dpf, the development of dopaminergic neurons of transgenic zebrafish Vmat:GFP (n=8) and the brain blood vessels development of transgenic zebrafish Fli1:GFP (n=8) were observed and recorded by fluorescence. At 5 dpf, the behavior of wild-type zebrafish AB (n=8) was analyzed by Zebrabox zebrafish behavior analyzer, and the expression of genes (α-syn、parkin、pink1)involved in Parkinsons desease(PD)in wild-type zebrafish AB (n=40) was detected by qRT-PCR. Compared with the control group, MPTP treatment group exhibited loss of dopaminergic neurons, damaged blood vessels in the brain, existence of PD-like behavior, and abnormal expression of PD-related genes. Compared with the MPTP treatment group, co-treatment groups reduced dopaminergic neurons loss and the damage of blood vessels in the brain, as well as relieved PD-like behavior and abnormal expression of PD-related genes, and these effects were strengthened with the increase of peony seed oil concentration. The results showed that peony seed oil microcapsule powder had anti-Parkinsons disease activity and was dose-dependent. |
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