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| Enzymatic synthesis of structured lipids rich in 1-oleoyl-2-palmitoyl-3-linoleylglycerol |
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| DOI：10.12166/j.zgyz.1003-7969/2020.08.014 |
| KeyWord:1-oleoyl-2-palmitoyl-3-linoleylglycerol structured lipids enzymatic acidolysis Lipozyme RM IM |
| FundProject:国家自然科学基金资助项目（31701559，31972035） |
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| Abstract: |
| Fractionated palm stearin was reacted with oleic acid and linoleic acid under the catalysis of Lipozyme RM IM to synthesize structured lipids rich in 1-oleoyl-2-palmitoyl-3-linoleylglycerol(OPL). The synthesis conditions were optimized by single factor experiment. The results showed that the optimal conditions were abtained as follows: molar ratio of palm stearin to oleic acid to linoleic acid 1∶ 7∶ 7; enzyme dosage 8%, reaction temperature 60 ℃, reaction time 6 h. The reaction was amplified 50 times under the optimal conditions, and the contents of OPL and sn-2 palmitic acid were 48.37% and 8470%, respectively. |
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