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| 酶法合成1-油酸-2-棕榈酸-3-亚油酸甘油三酯结构脂的研究 |
| Enzymatic synthesis of structured lipids rich in 1-oleoyl-2-palmitoyl-3-linoleylglycerol |
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| DOI：10.12166/j.zgyz.1003-7969/2020.08.014 |
| 中文关键词: 1-油酸-2-棕榈酸-3-亚油酸甘油三酯 结构脂 酶法酸解 Lipozyme RM IM |
| 英文关键词:1-oleoyl-2-palmitoyl-3-linoleylglycerol structured lipids enzymatic acidolysis Lipozyme RM IM |
| 基金项目:国家自然科学基金资助项目（31701559，31972035） |
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| 中文摘要: |
| 在Lipozyme RM IM脂肪酶的催化作用下，将分提后的棕榈硬脂与油酸和亚油酸反应，通过酶法酸解合成富含1-油酸-2-棕榈酸-3-亚油酸甘油三酯（OPL）的结构脂，通过单因素实验对酶法合成反应条件进行了优化。结果表明，合成OPL结构脂的最优反应条件为：棕榈硬脂、油酸、亚油酸摩尔比1∶ 7∶ 7，酶添加量8%，反应温度60 ℃，反应时间6 h。在最优条件下，进行50倍放大实验，合成产物中OPL和sn-2位棕榈酸的含量分别为48.37%和84.70%。 |
| 英文摘要: |
| Fractionated palm stearin was reacted with oleic acid and linoleic acid under the catalysis of Lipozyme RM IM to synthesize structured lipids rich in 1-oleoyl-2-palmitoyl-3-linoleylglycerol(OPL). The synthesis conditions were optimized by single factor experiment. The results showed that the optimal conditions were abtained as follows: molar ratio of palm stearin to oleic acid to linoleic acid 1∶ 7∶ 7; enzyme dosage 8%, reaction temperature 60 ℃, reaction time 6 h. The reaction was amplified 50 times under the optimal conditions, and the contents of OPL and sn-2 palmitic acid were 48.37% and 8470%, respectively. |
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