| The effects of algae-bacteria mixed culture (M), supplement of NaHCO3 culture(C), and the combination of both (MC) on the growth and lipid synthesis of Scenedesmus obliquus were studied. Four microorganisms separated from algae broth were mixed cultured with S. obliquus,then the best bacterial B1 for promoting growth of S. obliquus was selected and the optimal inoculation ratio was determined; the optimal NaHCO3mass concentration in supplement of NaHCO3 culture was determined; then the M, C and MC experiments were carried out,the growth indexes and lipid synthesis indexes of the culture methods were determined and compared with individual culture. The results showed that compared with the individual culture, the biomasses and lipid contents of S. obliquus by M, C and MC increased by 68.3%, 98.8%, 195.1% and 35.6%, 41.6%, 50.4%, respectively; and the C18∶ 1 contents in the lipid increased by 17.0%, 30.9% and 47.4%, respectively. Therefore, MC was more efficient than M and C, and MC had potential application value as a biodiesel raw material production mode. |
