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| 青海亚麻籽总酚含量测定及其抗氧化活性研究 |
| Determination of total phenol content and antioxidant activity of flaxseeds in Qinghai |
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| DOI：10.12166/j.zgyz.1003-7969/2020.08.024 |
| 中文关键词: 亚麻籽 总酚 超声辅助提取法 抗氧化活性 |
| 英文关键词:flaxseed total phenol ultrasound-assisted extraction antioxidant activity |
| 基金项目:青海省青年自然科学基金(2018-ZJ-970Q)；青海大学省部共建三江源生态与高原农牧业国家重点实验室自主课题（2019-ZZ-06） |
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| 中文摘要: |
| 以4种青海本地（贵德、互助、三合、民和）和3种分别来自甘肃、山西和内蒙古产地亚麻籽为原料，采用超声辅助提取法提取总酚，福林酚法测定总酚含量，并采用DPPH自由基清除能力实验评价其抗氧化活性。结果表明：超声辅助提取亚麻籽总酚的最佳工艺条件为固液比1∶ 17、超声温度40 ℃、超声时间15 min，在此条件下总酚含量为30.21 mg/g。青海贵德、互助、三合、民和的亚麻籽总酚含量为23.16～28.70 mg/g，DPPH自由基清除率为42.62%～51.06%，而山西、内蒙古、甘肃产亚麻籽总酚含量为19.97～22.38 mg/g，DPPH自由基清除率为41.84%～42.43%，表明青海亚麻籽总酚含量及抗氧化能力具有一定优势,具有较好的应用前景。 |
| 英文摘要: |
| Using four Qinghai flaxseeds and three flaxseeds from Shanxi, Inner Mongolia and Gansu as raw materials, total phenol were extracted by ultrasound-assisted extraction, the total phenol contents were determined by Folin method, and the antioxidant activity of total phenol was evaluated by DPPH free radical scavenging test. The results showed that the optimal process for ultrasound-assisted extraction of total phenol from flaxseeds was obtained as follows: solid-liquid ratio 1∶ 17, ultrasonic temperature 40 ℃, and ultrasonic time 15 min. Under these conditions, the total phenol content was 30.21 mg/g. The total phenol contents in the flaxseeds from Qinghai Guide, Huzhu, Sanhe and Minhe were 23.16-28.70 mg/g, and the DPPH free radical scavenging rates were 42.62%-51.06%, however, the total phenol contents of flaxseeds from Shanxi, Inner Mongolia and Gansu were 19.97-22.38 mg/g, and the DPPH free radical scavenging rates were 41.84%-42.43%, indicating that the total phenol content and antioxidant capacity in Qinghai flaxseed had certain advantages and had a good application prospect. |
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