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| Soxhlet extraction process, composition analysis and antioxidant activity of Iris lactea seed oil |
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| DOI： |
| KeyWord:Iris lactea seed oil extraction process response surface methodology GC-MS fatty acid composition unsaponifiable matter antioxidant activity |
| FundProject:国家自然科学基金青年基金（81403051）；青海省高端创新人才千人计划项目（2017年度）；青海省自然科学基金面上项目（2019-ZJ-904）；青海省科研基础条件创新平台专项项目(2020-ZJ-T05) |
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| Abstract: |
| The petroleum ether was used as the extraction solvent, extraction time, extraction temperature and ratio of material to liquid were selected as influencing factors, and the extraction conditions of Iris lactea seed oil (ILSO) were optimized by response surface methodology on the basis of single factor experiment with yield of ILSO as response value. The physicochemical properties, fatty acid composition and unsaponifiable matter components of ILSO were analyzed, and the antioxidant activities of ILSO were also studied. The results showed that the optimal extraction conditions of ILSO were obtained as follows: extraction time 6 h, extraction temperature 70 ℃, and ratio of material to liquid 1∶ 26. Under these conditions, the yield of ILSO reached 11.20%. The ILSO was yellow and transparent.The absorbance of the ILSO at 265 nm was 4.667, the unsaponifiable matter content was 137%, the saponification value was 185.2 mgKOH/g, the acid value was 0.038 mgKOH/g, the iodine value was 113.7 gI/100 g, and the peroxide value was 5.159 mmol/kg. The GC-MS results showed that the content of unsaturated fatty acids in ILSO was 84.71%, and the fatty acids were mainly composed of linoleic acid (45.32%), oleic acid (38.42%), palmitic acid (6.88%) and stearic acid (2.38%). The total content of sterols in unsaponifiable matters was as high as 66.19%, in which β-sitosterol content was 725.87 mg/kg, stigmasterol content was 1 231.55 mg/kg, and campesterol content was 410.50 mg/kg. The ILSO also contained a high concentration of antioxidant stigmasterol-5,24 (28) -diene-3β-ol. ILSO had good antioxidant activity with the IC50 of scavenging rate on DPPH free radical 19.86 mg/mL and the total antioxidant capacity determined by FRAP method 0.202 4 mmol/L (mass concentration of ILSO 280 mg/mL). |
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