| The antioxidant capacity of Elaeagnus mollis Diels seed oil extracted by five different processes (supercritical CO2 fluid extraction method, solvent extraction method, cold pressing method, hot pressing method and infrared hot pressing method) was evaluated by DPPH, ABTS and hydroxyl radical scavenging capacity, and PF reducing power, as well as the antioxidant capacity of the polar and non-polar fractions of the oil. In addition, the trace active components in Elaeagnus mollis Diels seed oil were analyzed, and the correlation analysis of the trace active components content and its antioxidant capacity was carried out. The results showed that except for the hydroxyl radical scavenging capacity, the antioxidant capacities on DPPH and ABTS and PF reducing power varied from the different oils, and the polar fractions of oils obtained by the five processes had the strongest antioxidant capacity, followed by the whole oil, and the non-polar fraction was the weakest. In the polar fraction of Elaeagnus mollis Diels seed oil, infrared hot pressing method had the highest DPPH scavenging capacity (1 080.35 μmolTE/100 g), cold pressing method had the highest hydroxyl radical scavenging capacity (38 272.82 μmolTE/100 g), while solvent extraction had the highest ABTS free radical scavenging capacity (2 000.76 μmolTE/100 g), and infrared hot pressing method had the highest Fe3+ reducing power (2 771.89 μmolTE/100 g). The correlation analysis showed that the bioactive components in Elaeagnus mollis Diels seed oil were significantly and positively correlated with the four kinds of antioxidant activity indexes(p <0.01). The essential antioxidant substances in Elaeagnus mollis Diels seed oil were γ-tocopherol, δ-tocopherol and lupeol. |
