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Purification of linoleic acid in Xinjiang seeding-watermelon seed oil by urea adduction
  
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KeyWord:seeding-watermelon seed oil  linoleic acid  urea adduction  gas chromatography
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HU Li 1.College of Life Science and Technology，Xinjiang University，Urumqi 830046，China； 
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Abstract:
      The linoleic acid in Xinjiang seeding-watermelon seed oil was purified by urea adduction.With the iodine value and yield of the product as evaluation indexes, the Box-Behnken response surface methodology was used to optimize the process on the basis of single factor experiment,and the optimal purification conditions were obtained as follows: adduction time 13 h, adduction temperature 4 ℃, ratio of volume of ethanol to mass of urea 4∶ 1, and mass ratio of urea to mixed fatty acids 3∶ 1.Under the optimal conditions, the iodine value of the product was 146.14 gI/100 g and the yield was 46.55%. The fatty acid composition of the product was analyzed by gas chromatography,and it was found that most of the saturated fatty acids had been removed after urea adduction and the content of linoleic acid increased significantly from 72.910% to 97.249%.
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