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| Hyperlipidemia induced by high-fat diet in rats and pathological changes in different organs |
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| DOI： |
| KeyWord:high-fat diet hyperlipidemia rat PD-1 immune inflammation |
| FundProject:山东省中医药科技发展计划项目（2019-0455）；济宁医学院大学生科研项目(JYXS2017KJ022)；济宁医学院校级教育科学研究项目（18066）；济宁医学院青年教师科研扶持基金（JY2017KJ046） |
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| Abstract: |
| In order to establish hyperlipidemia rats model by high-fat diet and observe expression of PD-1 in hyperlipidemia rats, forty male SD rats were divided into two groups of high-fat diet group and normal diet group and fed for twelve weeks with normal feed and high-fat feed, respectively. The liver index, spleen index and pancreas index were calculated. The blood glucose and serum triglyceride (TG), total cholesterol (T-CHO) and high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) concentrations were determined. HE staining was used to observe the pathological changes of rat liver, and PD-1 mRNA expressions in rat liver, spleen and pancreas were detected by RT-qPCR. The results showed that the liver index, spleen index and pancreas index in the high-fat diet group were significantly higher than those in the normal diet group. The blood glucose in the high-fat diet group was significantly increased, the serum levels of TG, T-CHO, HDL-C and LDL-C in the high-fat diet group were higher than those in the normal diet group, and the ratio of LDL-C to HDL-C was also significantly higher than that in the normal diet group. HE staining showed that there were obvious fat vacuoles in the liver tissue of rats fed with high-fat diet. The expressions of PD-1 mRNA in liver, spleen and pancreas tissue of rats fed with high-fat diet were significantly lower than that of rats fed with nomal diet, indicating that PD-1 might participate in the occurrence and development of hyperlipidemia. |
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