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| Phospholipids profile analysis of oilseeds by hydrophilic interaction chromatography-tandem mass spectrometry combined with reversed phase liquid chromatography-tandem mass spectrometry |
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| DOI： |
| KeyWord:HILIC Q-TOF-MS oilseed phospholipids |
| FundProject:国家自然科学基金（31872895）；国家食品科学与工程一流学科建设项目（JUFSTR20180202） |
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| Abstract: |
| Using ultra high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technology, a phospholipids profile analysis method combining with hydrophilic chromatography (HILIC) and reversed phase chromatography(RPLC) was established. The molecular species of the main phospholipids in eight oilseeds were comprehensively separated and detected, including PE, PC, PI, LPE and LPC. The results showed that the composition of PC and PE molecular species was generally similar among the eight oilseeds, but the composition of PI, LPC and LPE molecular species was significantly different. The contents of PI in rapeseed, Amygdalus pedunculata pall, and Plukenetia volubilis Linneo were higher than that in soybean. The main composition of PI in rapeseed was 16∶ 0-18∶ 2 and 18∶ 1-18∶ 2. In Amygdalus pedunculata pall, the main composition was 16∶ 0-18∶ 1 and the Plukenetia volubilis Linneo was mainly composed of 16∶ 0-18∶ 2 and 18∶ 2-18∶ 2. Cottonseed and flaxseed had higher lysophospholipid content and fatty acids were mainly linoleic acid for cottonseed, and oleic acid for flaxseed.The method had the items of rapidness, sensitiveness and accuracy, which could realize the simultaneously separation of various glycerophospholipids, lysophospholipids and their isomers. |
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