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| 高效液相色谱-蒸发光散射法分析海鲈肌肉磷脂组成 |
| Determination of phospholipids composition in Lateolabrax japonicus muscle by HPLC-ELSD |
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| DOI： |
| 中文关键词: 海鲈 磷脂 高效液相色谱蒸发光散射法（HPLC-ELSD） 脂肪酸 |
| 英文关键词:Lateolabrax japonicus phospholipids high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) fatty acid |
| 基金项目:“十二五”国家重点研发计划（2016YFD0400201-6）；中央级公益性科研院所基本科研业务费专项资金项目（2019TS16）；现代农业产业技术体系专项（CARS-47）；“扬帆计划”引进创新创业团队专项（2015YT02H109） |
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| 中文摘要: |
| 对海水鱼海鲈肌肉磷脂的脂肪酸组成进行了测定，并建立了利用高效液相色谱-蒸发光散射法（HPLC-ELSD）分析其磷脂组成的方法。采用Chromolith Performance-Si型正相硅胶色谱柱（100 mm×4.6 mm）进行磷脂组分分离，流动相为正己烷-异丙醇-乙酸体系，三元梯度洗脱，流速为1.5 mL/min，以各磷脂组分保留时间定性，峰面积定量。结果表明：海鲈肌肉磷脂不饱和脂肪酸含量为62.59%，EPA和DHA总含量达30.86%。采用HPLC-ELSD，磷脂组分分离效果良好，精密度高，变异系数均小于3%，平均回收率为86.00%~105.00%，相对标准偏差小于3.50%，此方法测定海鲈肌肉磷脂组成准确、高效，可运用于其他如海水鱼磷脂的分析检测。海鲈肌肉包含6种磷脂组分，其中磷脂酰乙醇胺（PE）含量最高，磷脂酰胆碱（PC）和磷脂酰肌醇（PI）次之。 |
| 英文摘要: |
| The fatty acid composition of phospholipids in seawater fish Lateolabrax japonicus muscle was studied. The method for analyzing the phospholipids composition by high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)was established. Chromolith Performance-Si type positive phase silica gel column(100 mm × 4.6 mm)was used as separation column, and n-hexane-isopropanol-acetic acid was used as mobile phase system with three element gradient elution and flow rate 1.5 mL/min. Each phospholipids component was qualified by retention time and quantified by peak area.The results indicated that the content of unsaturated fatty acids in Lateolabrax japonicus muscle phospholipids was 62.59%, and the total content of EPA and DHA was 30.86%. Each phospholipids component was separated well by HPLC-ELSD.The method had high precision, and the coefficient of variation was less than 3%. The average recovery rate was in the range of 86.00%-105.00%, and the relative standard deviation was less than 3.50%. The method was accurate and efficient for the determination of phospholipids composition in Lateolabrax japonicus muscle, and was suitable for the determination of phospholipids in seawater fish. Six kinds of phospholipids components were detected in Lateolabrax japonicus muscle. Among them, the content of phosphatidylethanolamine (PE) was the highest, followed by phosphatidylcholines(PC) and phosphatidylinositol(PI). |
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