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| 蚕豆蛋白酶解物分离纯化及降胆固醇活性 |
| Separation and purification of broad bean protein hydrolysate andits cholesterol lowering activity |
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| DOI： |
| 中文关键词: 蚕豆 蚕豆蛋白酶解物 胆固醇 凝胶过滤色谱 分离纯化 |
| 英文关键词:broad bean broad bean protein hydrolysate cholesterol gel filtration chromatography separation and purification |
| 基金项目: |
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| 中文摘要: |
| 采用凝胶过滤色谱对经过大孔吸附树脂纯化的蚕豆蛋白酶解物进行分离纯化，以期得到高降胆固醇活性的酶解物组分。结果表明：单因素试验得到蚕豆蛋白酶解物凝胶过滤色谱最佳分离纯化工艺条件为Sep G-10、G-25葡聚糖凝胶为柱填充材料，10 mg/mL的酶解液上样量4 mL，洗脱剂为去离子水，洗脱流速1 mL/min。凝胶过滤色谱分离纯化后得到F1~F6 6个蚕豆蛋白酶解物组分；与10 mg/mL考来烯胺散阳性对照比较， F3、F6组分对3种胆酸盐（胆酸钠、甘氨胆钠酸、牛磺胆酸钠）抑制率均高于阳性对照，其中F6组分的相对抑制率最高，分别为（274.98±0.19）%、（140.22±0.20）%、（130.99±0.22）%。 |
| 英文摘要: |
| The broad bean protein hydrolysate purified by macroporous adsorption resin was separated and purified by gel filtration chromatography to obtain broad bean protein hydrolysate with cholesterol lowering activity. The results showed that the optimal separation and purification conditions of the broad bean protein hydrolysate by single factor experiment were obtained as follows: with Sep G-10 and G-25 dextran gel as the column filling material, loading amount of 10 mg/mL enzymatic hydrolysate 4 mL, deionized water as mobile phase, elution speed 1 mL/min. After separated and purified by the gel filtration chromatography, the components of F1-F6 six broad bean protein hydrolysates were obtained. Compared with the 10 mg/mL of coletenamine powder positive control, the inhibitory rates of F3 and F6 components to three kinds of cholate salts(sodium cholate, soldium glycocholate, sodium taurocholate) were higher than those of the positive control. The relative inhibition rate of F6 component was the highest, which was (274.98 ± 0.19)%, (140.22 ± 0.20)% and (130.99±0.22)% respectively. |
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