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| Preparation of eleostearic acid by enzymatic hydrolysis of tung oil |
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| DOI： |
| KeyWord:tung oil α-eleostearic acid enzymatic hydrolysis response surface methodology |
| FundProject:湖南省教育厅科技计划项目（17A174） |
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| Abstract: |
| In order to explore the reasonable process for preparing eleostearic acid by enzymatic hydrolysis of tung oil, the effects of hydrolysis temperature, amount of water and amount of enzyme on hydrolysis rate of tung oil were studied by single factor experiment. Then with hydrolysis rate of tung oil and α-eleostearic acid retention rate as indexes, the enzymatic hydrolysis conditions of tung oil were optimized using response surface methodology. The results showed that the optimal process parameters for preparing eleostearic acid by enzymatic hydrolysis of tung oil were obtained as follows: hydrolysis temperature 45 ℃, amount of water 22% and amount of enzyme 0.5%. Under these conditions, the hydrolysis rate of tung oil was 90.15% and the α-eleostearic acid retention rate was 98.26%. |
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