Ten representative Juglans sigillata samples were collected from three main producing areas of Xinjiang, Yunnan and Tibet to prepare Juglans sigillata oil, and the fatty acid composition,contents of total phenol,tocopherol and phytosterol,and oxidative stability of Juglans sigillata oil were determined, also the composition characteristics of Juglans sigillata oil was analyzed by chemometrics. The results showed that linoleic acid (54.84%-66.97%), oleic acid (15.88%-26.04%), linolenic acid (6.82%-12.25%) and palmitic acid (5.12%-7.62%) were the main fatty acid of Juglans sigillata oil, the contents of tocopherol, phytosterol and total phenol were 176.89 -832.86 mg/kg, 530.96-1 095.47 mg/kg and 1.42-15.84 mg/kg, respectively,and the oxidative stability indexes (OSI) were 0.55-3.39 h. Based on multivariate linear regression analysis, it was found that linoleic acid, linolenic acid, γ-tocopherol and total phenol were the main components that affected the oxidation stability of Juglans sigillata oil |