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| 以葵花籽油为底物生物转化共轭亚油酸双歧杆菌的筛选 |
| Screening of conjugated linoleic acid producing bifidobacteria with sunflower seed oil as substrate |
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| DOI： |
| 中文关键词: 共轭亚油酸 双歧杆菌 葵花籽油 亚油酸 快速扫描 |
| 英文关键词:conjugated linoleic acid bifidobacteria sunflower seed oil linoleic acid rapid screening |
| 基金项目:国家自然科学基金（31801521）；江苏高校品牌专业建设工程项目（PPZY2015A052） |
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| 中文摘要: |
| 运用快速扫描法，筛选了10株可利用游离态亚油酸为底物高产共轭亚油酸的双歧杆菌菌株；然后以富含非游离态亚油酸的葵花籽油为底物，与初筛得到的高产菌株共培养后检测反应体系中共轭亚油酸的含量。结果表明，部分短双歧杆菌可将葵花籽油转化为共轭亚油酸，其中短双歧杆菌FFJND12M6的转化率最高，可达14.35%。 |
| 英文摘要: |
| Ten bifidobacteria strains with high yield of conjugated linoleic acid using free linoleic acid as substrate was screened via rapid scanning approach. Then, using sunflower seed oil rich in non-free linoleic acid as substrate, the high-yield strains obtained from the initial screening was cultured and the content of conjugated linoleic acid in the reaction system was detected. The results showed that Bifidobacterium breve could generally convert sunflower seed oil to conjugated linoleic acid, and Bifidobacterium breve FFJND12M6 showed the highest conversion rate, up to 14.35%. |
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