| The protective effect of walnut oil (WO) on dextran sulfate sodium (DSS) induced colitis in mice was investigated. Kunming mice were randomly divided into four groups: control group (Con), DSS group, DSS+WO group and WO group. Serum proinflammatory factors, antioxidant enzyme activity in colon tissue, Nod-like receptor pyrin domain 3 (NLRP3) and related mRNA levels were detected. The results showed that, compared with Con group, the contents of serum IL-1β and IL-6, transcription levels of NLRP3, Caspase-1 and IL-1β mRNA and NLRP3 protein expression level in colon of DSS group significantly increased (p<0.01), and activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in colon significantly decreased (p<0.01). Compared with DSS group, the contents of serum IL-1β and IL-6, transcription levels of NLRP3 and Caspase-1 mRNA and NLRP3 protein expression level in colon of DSS+WO group significantly decreased (p<001), and the activities of SOD, GSH-Px and CAT in colon significantly increased (p<0.01). Walnut oil might play a protective role in DSS induced colitis in mice by enhancing the antioxidant capacity of the colon and possibly reducing the expression level of related inflammatory factors by inhibiting the transcription level of NLRP3 inflammasome pathway genes. |
