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| 生物转化共轭亚油酸乳杆菌的筛选 |
| Screening of Lactobacillus with high conjugated linoleic acid production capacity |
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| DOI： |
| 中文关键词: 共轭亚油酸 乳杆菌 酶标仪 气相色谱质谱联用 |
| 英文关键词:conjugated linoleic acid Lactobacillus enzyme-labeled instrument gas chromatography-mass spectrometry |
| 基金项目:国家自然科学基金项目（31801521）;江苏高校品牌专业建设工程项目（PPZY2015A052） |
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| 中文摘要: |
| 以选定的乳杆菌为对象，系统性地探究其生物转化共轭亚油酸（CLA）的规律，并筛选得到高产菌株。首先用酶标仪快速检测法，对5个种属76株乳杆菌生物转化CLA的能力进行了分析，然后利用GC-MS进一步分析高产菌株生物转化CLA产物异构体组成。结果显示：植物乳杆菌大部分可生物转化CLA，但菌株间转化能力存在较大差异；副干酪乳杆菌、干酪乳杆菌和卷曲乳杆菌普遍具备生物转化CLA的能力，但转化能力不高；唾液乳杆菌普遍不具备生物转化CLA的能力。筛选得到3株高产菌株，其中植物乳杆菌CCFM241 CLA的转化能力最高，CLA转化率为16.35%，转化产物存在3种CLA异构体，以t9,t11-CLA为主。 |
| 英文摘要: |
| ：Taking selected Lactobacillus as the object, the law of its biotransformation of conjugated linoleic acid (CLA) were systematically explored, and high-yield strains were screened.The biotransformation ability of 76 strains of Lactobacillus from 5 species to CLA were analyzed by enzyme-labeled instrument, then the isomer composition of CLA obtained by high-yield strains was analyzed by GC-MS.The results showed that most of Lactobacillus plantarum could biotransform to CLA linoleic acid, but there were great differences among strains. Lactobacillus paracasei, Lactobacillus casei and Lactobacillus crispatus generally had the biotransformation ability to CLA, but the transformation ability was not high. Lactobacillus salivarius generally did not had the ability to biotransform to CLA.In addition, three strains with high-yield CLA were screened, among which Lactobacillus plantarum CCFM241 had the highest transformation ability, with a conversion rate of 16.35%, and there were three CLA isomers in the converted products, mainly was t9,t11-CLA. |
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