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| Screening of Lactobacillus with high conjugated linoleic acid production capacity |
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| DOI： |
| KeyWord:conjugated linoleic acid Lactobacillus enzyme-labeled instrument gas chromatography-mass spectrometry |
| FundProject:国家自然科学基金项目（31801521）;江苏高校品牌专业建设工程项目（PPZY2015A052） |
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| Abstract: |
| ：Taking selected Lactobacillus as the object, the law of its biotransformation of conjugated linoleic acid (CLA) were systematically explored, and high-yield strains were screened.The biotransformation ability of 76 strains of Lactobacillus from 5 species to CLA were analyzed by enzyme-labeled instrument, then the isomer composition of CLA obtained by high-yield strains was analyzed by GC-MS.The results showed that most of Lactobacillus plantarum could biotransform to CLA linoleic acid, but there were great differences among strains. Lactobacillus paracasei, Lactobacillus casei and Lactobacillus crispatus generally had the biotransformation ability to CLA, but the transformation ability was not high. Lactobacillus salivarius generally did not had the ability to biotransform to CLA.In addition, three strains with high-yield CLA were screened, among which Lactobacillus plantarum CCFM241 had the highest transformation ability, with a conversion rate of 16.35%, and there were three CLA isomers in the converted products, mainly was t9,t11-CLA. |
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