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| ZIF-8原位合成法固定化脂肪酶CRL |
| Immobilization of lipase CRL on ZIF-8 by in situ synthesis |
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| DOI： |
| 中文关键词: 褶皱假丝酵母脂肪酶(CRL) ZIF-8 原位合成法 固定化 |
| 英文关键词:lipase from Candida rugosa(CRL) ZIF-8 in situ synthesis immobilization |
| 基金项目:国家自然科学基金（21966007）；广西自然科学基金（2018GXNSFAA281278） |
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| 中文摘要: |
| 以ZIF-8为固定化载体，通过原位合成法固定褶皱假丝酵母脂肪酶（CRL），研究了不同固定化条件对固定化酶活力的影响以及固定化酶的稳定性,并对固定化酶进行了结构表征。结果表明：在10 mL 40 mmol/L硝酸锌溶液、10 mL 160 mmol/L 2-甲基咪唑溶液、2-甲基咪唑溶液初始pH 8、酶添加量2.75 mg、-48 ℃冷冻干燥条件下,获得的固定化酶CRL@ZIF-8蛋白回收率为93%，比活力为1.08 U/mg；底物的分子大小对固定化酶的催化性能有明显影响，在以相对小分子的乙酸对硝基苯酯为底物时，重复使用9次后固定化酶仍保持72.8%的相对活力；通过对固定化酶进行场发射扫描电镜和氮气吸附脱附表征，表明脂肪酶CRL在载体表面和内部均有分布，固定化酶颗粒粒径约400 nm。 |
| 英文摘要: |
| ZIF-8 was employed as the support for the immobilization of lipase from Candida rugosa (CRL) through in situ synthesis. The effects of different immobilization conditions on the activity of immobilized lipase and the stability of immobilized lipase were studied, and the structure of the immobilized lipase was characterized. The results showed that under the conditions of 10 mL 40 mmol/L zinc nitrate, 10 mL 160 mmol/L 2-methylimidazol, initial pH 8.0, lipase dosage 2.75 mg and freeze drying at-48 ℃,the protein recovery rate and specific activity of the immobilized lipase(CRL@ZIF-8)were 93% and 1.08 U/mg, respectively. Besides, it was demonstrated that the size of substrates had a significant impact on the catalytic property of the immobilized lipase. With p-nitrobenzene acetate as the substrate, the relative activity of immobilized lipase was 72.8% after nine times of reuse. According to the field emission scanning electron microscopy (FSEM) and BET, the lipase CRL was found to embed both on the surface and in the interior of ZIF-8. The diameter of the immobilized lipase was about 400 nm. |
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