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| Immobilization of lipase CRL on ZIF-8 by in situ synthesis |
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| DOI： |
| KeyWord:lipase from Candida rugosa(CRL) ZIF-8 in situ synthesis immobilization |
| FundProject:国家自然科学基金（21966007）；广西自然科学基金（2018GXNSFAA281278） |
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| Abstract: |
| ZIF-8 was employed as the support for the immobilization of lipase from Candida rugosa (CRL) through in situ synthesis. The effects of different immobilization conditions on the activity of immobilized lipase and the stability of immobilized lipase were studied, and the structure of the immobilized lipase was characterized. The results showed that under the conditions of 10 mL 40 mmol/L zinc nitrate, 10 mL 160 mmol/L 2-methylimidazol, initial pH 8.0, lipase dosage 2.75 mg and freeze drying at-48 ℃,the protein recovery rate and specific activity of the immobilized lipase(CRL@ZIF-8)were 93% and 1.08 U/mg, respectively. Besides, it was demonstrated that the size of substrates had a significant impact on the catalytic property of the immobilized lipase. With p-nitrobenzene acetate as the substrate, the relative activity of immobilized lipase was 72.8% after nine times of reuse. According to the field emission scanning electron microscopy (FSEM) and BET, the lipase CRL was found to embed both on the surface and in the interior of ZIF-8. The diameter of the immobilized lipase was about 400 nm. |
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