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| 脂肪酶-蜗牛酶提取茶籽多糖及其抗氧化作用 |
| Extraction of Camellia oleifera seed polysaccharides by lipase-snailase andits antioxidant effect |
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| DOI： |
| 中文关键词: 茶籽多糖 脂肪酶 蜗牛酶 抗氧化 |
| 英文关键词:Camellia oleifera seed polysaccharides (COSP) lipase snailase antioxidation |
| 基金项目:江西省教育厅科学技术研究项目（GJJ161055） |
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| 中文摘要: |
| 摘要：以油茶籽饼为原料，优化脂肪酶-蜗牛酶提取茶籽多糖的条件，探讨酶提茶籽多糖的抗氧化作用。通过单因素试验和正交试验分析酶解温度和pH、酶解时间、酶添加量、脂肪酶和蜗牛酶质量比对茶籽多糖得率的影响；测定和比较酶提茶籽多糖与水提茶籽多糖对O-2·、DPPH·、·OH、ABTS+·4种自由基的清除率；采用Schaal烘箱法研究酶提茶籽多糖对油脂的抗氧化作用。结果表明：脂肪酶-蜗牛酶提取茶籽多糖的最佳工艺条件为酶解温度35 ℃、pH 7.0、酶解时间2.5 h、酶添加量2.0%、脂肪酶和蜗牛酶质量比2∶ 3，在此条件下茶籽多糖得率可达5.83%；酶提茶籽多糖的抗氧化作用强于水提茶籽多糖。酶提茶籽多糖具有作为油脂抗氧化剂的潜质。 |
| 英文摘要: |
| The conditions of extracting Camellia oleifera seed polysaccharides (COSP) from Camellia oleifera seed cake by lipase-snailase were optimized and the antioxidant effect of COSP was investigated. The effects of enzymolysis temperature and pH, enzymolysis time, enzyme dosage, mass ratio of lipase to snailase on yield of COSP were analysed by single factor experiment and orthogonal experiment. The scavenging rates of COSP extracted by lipase-snailase and water on O-2·, DPPH·, ·OH and ABTS+· were determined and compared. The antioxidant effects of COSP extracted by lipase-snailase on oils were investigated by Schaal oven method. The results showed that the optimal conditions of COSP extracted by lipase-snailase were obtained as follows: enzymolysis temperature 35 ℃, pH 7.0, enzymolysis time 2.5 h, enzyme dosage 2.0% and mass ratio of lipase to snailase 2∶ 3. Under these conditions, the yield of COSP was 5.83%. The antioxidant effect of COSP extracted by lipase-snailase was better than that of COSP extracted by water. COSP extracted by lipase-snailase had potential as antioxidant for oils. |
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