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| Extraction of Camellia oleifera seed polysaccharides by lipase-snailase andits antioxidant effect |
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| DOI： |
| KeyWord:Camellia oleifera seed polysaccharides (COSP) lipase snailase antioxidation |
| FundProject:江西省教育厅科学技术研究项目（GJJ161055） |
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| Abstract: |
| The conditions of extracting Camellia oleifera seed polysaccharides (COSP) from Camellia oleifera seed cake by lipase-snailase were optimized and the antioxidant effect of COSP was investigated. The effects of enzymolysis temperature and pH, enzymolysis time, enzyme dosage, mass ratio of lipase to snailase on yield of COSP were analysed by single factor experiment and orthogonal experiment. The scavenging rates of COSP extracted by lipase-snailase and water on O-2·, DPPH·, ·OH and ABTS+· were determined and compared. The antioxidant effects of COSP extracted by lipase-snailase on oils were investigated by Schaal oven method. The results showed that the optimal conditions of COSP extracted by lipase-snailase were obtained as follows: enzymolysis temperature 35 ℃, pH 7.0, enzymolysis time 2.5 h, enzyme dosage 2.0% and mass ratio of lipase to snailase 2∶ 3. Under these conditions, the yield of COSP was 5.83%. The antioxidant effect of COSP extracted by lipase-snailase was better than that of COSP extracted by water. COSP extracted by lipase-snailase had potential as antioxidant for oils. |
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