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| Extraction, purification, classific preparation and antioxidant activities of polysaccharides from peony leaves |
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| DOI： |
| KeyWord:peony leaf polysaccharides extraction and purification in vitro antioxidant activity |
| FundProject:西安医学院2018年配套基金项目（2018PT63）；西安医学院2018年青年科研基金项目（2018QN12）；陕西高校青年创新团队建设项目（陕教\[2019\]90号）；陕西省科技厅一般项目（2017SF-270） |
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| Abstract: |
| A method for extracting, isolating and purifing polysaccharides from peony leaves was established, and four polysaccharides from peony leaves（PW-25、PW-45、PW-65、PW-85）were obtained by ethanol graded precipitation method with 25%、45%、65% and 85% ethanol. The spectral characteristics of the four polysaccharides were determined by FTIR and the in vitro antioxidant activities were studied by DPPH free radical and hydroxyl free radical scavenging experiments. The results showed that the polysaccharides from peony leaves were isolated and purified by a combined method of ZTC1+1-II natural clarifying agents for removing protein and decolorization with hydrogen peroxide, and under this method, the removal rates of protein and pigment and retention rate of polysaccharides in crude polysaccharides from peony leaves were 89.66%, 79.35% and 61.41% respectively; the purity of polysaccharides PW-25 was 91.23%, and its yield was 1.29%; four polysaccharides from peony leaves showed certain in vitro antioxidant activities, and the IC50 of PW-25 by scavenging DPPH free radical and hydroxyl free radical were 15.36、6577 μg/mL respectively, which were slightly worse than VC（IC50 7.89、46.46 μg/mL）.The results could provide foundation for the development and application of polysaccharides from peony leaves. |
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